Zernike phase contrast in scanning microscopy with X-rays.

Nature Physics
Christian HolznerC Jacobsen

Abstract

Scanning X-ray microscopy focuses radiation to a small spot and probes the sample by raster scanning. It allows information to be obtained from secondary signals such as X-ray fluorescence, which yields an elemental mapping of the sample not available in full-field imaging. The analysis and interpretation from these secondary signals can be considerably enhanced if these data are coupled with structural information from transmission imaging. However, absorption often is negligible and phase contrast has not been easily available. Originally introduced with visible light, Zernike phase contrast(1) is a well-established technique in full-field X-ray microscopes for visualization of weakly absorbing samples(2-7). On the basis of reciprocity, we demonstrate the implementation of Zernike phase contrast in scanning X-ray microscopy, revealing structural detail simultaneously with hard-X-ray trace-element measurements. The method is straightforward to implement without significant influence on the resolution of the fluorescence images and delivers complementary information. We show images of biological specimens that clearly demonstrate the advantage of correlating morphology with elemental information.

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Citations

Oct 20, 2012·Analytical and Bioanalytical Chemistry·Stefan Vogt, Martina Ralle
Jul 20, 2012·Annual Review of Marine Science·Benjamin S Twining, Stephen B Baines
Feb 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Junjing DengChris J Jacobsen
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May 18, 2018·Journal of Biophotonics·Hsi-Hsun ChenYuan Luo
Oct 12, 2018·Microscopy·Wataru Yashiro
Apr 22, 2017·Microscopy·Atsushi Momose
Mar 14, 2019·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Masao KimuraKen'ichi Kimijima
Dec 25, 2013·Journal of Synchrotron Radiation·Young Pyo HongChris Jacobsen
Jul 3, 2015·Journal of Synchrotron Radiation·Heng ChenZiyu Wu
Dec 24, 2011·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Suzana Topuzoski, Ljiljana Janicijevic
May 31, 2014·Optics Letters·Marco EndrizziAlessandro Olivo
Feb 14, 2015·Physical Review Letters·M BartelsT Salditt
Feb 13, 2016·Optics Letters·Ismo VartiainenAlke Meents
Mar 30, 2020·Scientific Reports·Ziyao Lyu, Changshun Wang
Apr 6, 2021·Frontiers in Neuroscience·Paulla Vieira RodriguesMatheus de Castro Fonseca
Apr 16, 2021·Optics Letters·P Villanueva-PerezS Bajt
May 7, 2021·Journal of Applied Crystallography·Ming DuChris Jacobsen

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