Zerumbone protects INS-1 rat pancreatic beta cells from high glucose-induced apoptosis through generation of reactive oxygen species

Biochemical and Biophysical Research Communications
Changyin WangZhaofeng Han

Abstract

The aim of this study is to explore the effect of zerumbone, a natural sesquiterpene isolated from Zingiber zerumbet Smith, on high glucose-induced cytotoxicity in pancreatic β cells. INS-1 rat pancreatic β cells were treated with 33 mM glucose with or without different concentrations of zerumbone and cell viability and apoptosis were assessed. The involvement of reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) signaling in the action of zerumbone was examined. Notably, zerumbone significantly (P < 0.05) prevented the reduction of cell viability induced by high glucose. Such protection was in a concentration-dependent fashion up to 60 μM of zerumbone. Annexin-V/propidium iodide staining analysis showed that zerumbone impaired the apoptotic response of high glucose-treated INS-1 cells, which was coupled with a significant decline in cleaved caspase-3 and caspase-9. Pretreatment with the ROS inhibitor N-acetylcysteine abrogated the phosphorylation of p38 and JNK induced by high glucose. Zerumbone significantly (P < 0.05) decreased the generation of ROS and the phosphorylation of p38 and JNK MAPKs in high glucose-treated INS-1 cells. Pharmacological activation of p38 and JNK with anisomycin reversed the an...Continue Reading

References

Jan 1, 1994·Documenta Ophthalmologica. Advances in Ophthalmology·K R Hightower
Nov 30, 2000·Biochemical and Biophysical Research Communications·B Törocsik, J Szeberényi
Dec 28, 2002·Diabetes·Alexandra E ButlerPeter C Butler
Feb 10, 2004·The Journal of Biological Chemistry·James F Curtin, Thomas G Cotter
Apr 21, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michelle L HerdmanKinsley Kelley Kiningham
Jan 19, 2010·Biochimica Et Biophysica Acta·Eun Kyung Kim, Eui-Ju Choi
Feb 4, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Adel S Al-ZubairiM M Syam
Mar 29, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Songyan ZhangYu Liu
Aug 14, 2012·Molecular and Cellular Endocrinology·Mohammed BensellamJean-Christophe Jonas
Mar 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Thing-Fong TzengI-Min Liu
May 10, 2013·Molecular Biology Reports·Luis A Flores-LópezClara Ortega-Camarillo
Oct 5, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Haitao ShenXianghong Zhang
Jul 21, 2014·Breast Cancer Research and Treatment·Anuradha SehrawatShivendra V Singh
Dec 2, 2014·International Journal of Molecular Medicine·Jong Seok LeeEock Kee Hong

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Citations

Oct 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katayoon KalantariRosfarizan Mohamad
Jul 18, 2017·Food & Function·Md Areeful HaqueSyed Nasir Abbas Bukhari
Apr 4, 2019·International Journal of Molecular Medicine·Shengyu LiYanna Shen
May 16, 2017·International Immunopharmacology·Jong Won KimBumseok Kim
Apr 10, 2021·Archives of Physiology and Biochemistry·Li ZhangZhenzuo Li

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