Zerumbone Suppresses Angiogenesis in HepG2 Cells through Inhibition of Matrix Metalloproteinase-9, Vascular Endothelial Growth Factor, and Vascular Endothelial Growth Factor Receptor Expressions

Pharmacognosy Magazine
Nozlena Abdul SamadYeap Swee Keon

Abstract

Due to increase in the number of patients with impaired immunity, the incidence of liver cancer has increased considerably. The aim of this study is the investigation thein vitroanticancer effect of zerumbone (ZER) on hepatocellular carcinoma (HCC). The anticancer mechanism of ZER was determined by the rat aortic ring, human umbilical vein endothelial cells (HUVECs) proliferation, chorioallantoic membrane, cell migration, and proliferation inhibition assays. Our results showed that ZER reduced tube formation by HUVECs effectively inhibits new blood vessel and tissue matrix formation. Western blot analysis revealed that ZER significantly (P< 0.05) decreased expression of molecular effectors of angiogenesis, the matrix metalloproteinase-9, vascular endothelial growth factor (VEGF), and VEGF receptor proteins. We found that ZER inhibited the proliferation and suppressed migration of HepG2 cell in dose-dependent manner. Statistical analyses were performed according to the Statistical Package for Social Science (SPSS) version 17.0. The data were expressed as the mean ± standard deviation and analyzed using a one-way analysis of variance. AP< 0.05 was considered statistically significant. The study for the first time showed that ZER ...Continue Reading

Citations

Feb 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sosmitha GirisaAjaikumar B Kunnumakkara

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BETA
X-ray
Assay
protein assay

Software Mentioned

Image J
Leica QWin

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