Zeylenone Induces Mitochondrial Apoptosis and Inhibits Migration and Invasion in Gastric Cancer

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Shuxian YangLi Cao

Abstract

The mortality of gastric cancer (GC) is increasing due to its high rates of recurrence and metastasis. Zeylenone (Zey), a type of naturally occurring cyclohexene oxide, was demonstrated to be effective in cancer patients. The aim of this study is to explore the anti-cancer effect of Zey against gastric cancer both in vitro and in vivo, as well as the underlying mechanisms. We found that Zey inhibited gastric tumor growth, as demonstrated by in vitro gastric cancer cell lines and in a human gastric cancer xenograft mouse model. Furthermore, Zey induced substantial apoptosis through a mitochondrial apoptotic pathway, involving mitochondrial transmembrane potential loss, caspase-3 activation, anti-apoptotic protein downregulation, and pro-apoptotic protein upregulation. Notably, we revealed for the first time that Zey suppressed invasion and migration by wound healing and transwell chamber assays. Through Western blotting, we further explored the potential mechanism of Zey's anti-cancer activity. We found that Zey downregulated the expression of matrix metalloproteinase 2/9 (MMP 2/9) and inhibited the phosphorylation of AKT and ERK. In short, Zey, which induced mitochondrial apoptosis and inhibited proliferation, migration, and in...Continue Reading

References

Nov 21, 1998·Gut·G I MurrayJ E Fothergill
May 11, 2002·Nature Reviews. Cancer·Livio Trusolino, Paolo M Comoglio
Dec 18, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Seon Young NamByung Lan Lee
May 3, 2005·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Steven C CunninghamRichard D Schulick
Sep 20, 2005·Chemico-biological Interactions·Pei-Ni ChenShu-Chen Chu
May 26, 2006·Nature Reviews. Cancer·Patrick Mehlen, Alain Puisieux
Feb 20, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Salah-Eddin Al-BatranMichael Scholz
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Rebecca C TaylorSeamus J Martin
Jul 22, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Ahmedin JemalElizabeth M Ward
Mar 15, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayF Bray
Mar 4, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia-Hui ChuXian-Jun Qu
Mar 13, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Parisa KarimiFarin Kamangar
Mar 22, 2014·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ana FerroNuno Lunet
Jul 9, 2014·Cancers·Tasuku Matsuoka, Masakazu Yashiro
May 10, 2016·Lancet·Eric Van CutsemHans Prenen
Mar 10, 2017·Cellular and Molecular Gastroenterology and Hepatology·Bryson W Katona, Anil K Rustgi
Mar 31, 2017·Oncology Letters·Zhengrong ChenChungen Xing
Jan 7, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Boshun WanHonggeng Guan

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Citations

Feb 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto Fabiani
Jul 23, 2020·Acta Crystallographica. Section E, Crystallographic Communications·Theerachart LeepasertTanwawan Duangthongyou

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Methods Mentioned

BETA
xenograft
flow cytometry
Assay

Software Mentioned

ImageJ
GraphPad Prism
Image J

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