Zika virus dysregulates human Sertoli cell proteins involved in spermatogenesis with little effect on tight junctions

PLoS Neglected Tropical Diseases
Mahamud-Ur RashidKevin M Coombs

Abstract

Zika virus (ZIKV), a neglected tropical disease until its re-emergence in 2007, causes microcephaly in infants and Guillain-Barré syndrome in adults. Its re-emergence and spread to more than 80 countries led the World Health Organization in 2016 to declare a Public Health Emergency. ZIKV is mainly transmitted by mosquitos, but can persist in infected human male semen for prolonged periods and may be sexually transmitted. Testicular Sertoli cells support ZIKV replication and may be a reservoir for persistent ZIKV infection. Electrical impedance analyses indicated ZIKV infection rapidly disrupted Vero cell monolayers but had little effect upon human Sertoli cells (HSerC). We determined ZIKV-induced proteomic changes in HSerC using an aptamer-based multiplexed technique (SOMAscan) targeting >1300 human proteins. ZIKV infection caused differential expression of 299 proteins during three different time points, including 5 days after infection. Dysregulated proteins are involved in different bio-functions, including cell death and survival, cell cycle, maintenance of cellular function, cell signaling, cellular assembly, morphology, movement, molecular transport, and immune response. Many signaling pathways important for maintenance o...Continue Reading

References

Jan 1, 1987·Archives of Andrology·V SantiemmaA Fabbrini
Mar 1, 1985·Journal of Reproduction and Fertility·N H JutteV Hansson
Sep 15, 1994·The Biochemical Journal·J MalmB Dahlbäck
Sep 24, 1998·The Journal of Clinical Endocrinology and Metabolism·F H PierikR F Weber
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
Jan 5, 2002·Molecular Human Reproduction·Ken-ichi Shimokawa KiSatoru Kaneko
Jan 12, 2002·The American Journal of Pathology·Virve PentikäinenLeo Dunkel
Jan 22, 2004·Journal of Cell Science·Ching-Hang WongC Yan Cheng
Apr 1, 1997·Cell Death and Differentiation·L DunkelK Erkkilä
Feb 15, 1963·Science·A Blanco, W H Zinkham
Jan 8, 2008·Molecular and Cellular Endocrinology·Tal Almog, Zvi Naor
Feb 8, 2008·Reproductive Biology and Endocrinology : RB&E·Keisuke AbéShin-ichi Abé
Aug 1, 2008·American Journal of Physiology. Cell Physiology·Tadayuki OshimaTakashi Joh
Mar 24, 2009·Trends in Molecular Medicine·Michelle W M LiC Yan Cheng
Jul 30, 2009·American Journal of Physiology. Endocrinology and Metabolism·María F RieraSelva B Cigorraga
Oct 31, 2009·Journal of Andrology·Marilena D Papaioannou, Serge Nef
Aug 13, 2010·Journal of Virology·Kevin M CoombsEarl G Brown
May 3, 2011·Emerging Infectious Diseases·Brian D FoyRobert B Tesh
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhiyv NiuRalph L Brinster
Apr 27, 2012·Physiological Reviews·Jon M Oatley, Ralph L Brinster
Feb 5, 2013·Cell Stress & Chaperones·Lan-Juan ZhaoZhong-Tian Qi
Oct 17, 2013·Molecular and Cellular Endocrinology·M RegueiraS B Meroni
Nov 29, 2013·International Journal of Cancer. Journal International Du Cancer·Xiangli JiangXiubao Ren
May 6, 2014·Frontiers in Endocrinology·Giulia GuerrieroGaetano Ciarcia
Dec 23, 2015·Reproductive Biology and Endocrinology : RB&E·Zoë L VincentAnna P Ponnampalam

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Citations

Apr 30, 2021·Seminars in Cell & Developmental Biology·Rachel L WashburnJannette M Dufour
Jun 4, 2021·Frontiers in Microbiology·Daniel P StrangeSaguna Verma
Jul 10, 2021·World Journal of Microbiology & Biotechnology·Tholasi Nadhan NavienMarimuthu Citartan

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Methods Mentioned

BETA
ELISA
Protein Assay

Software Mentioned

SOMA
SOMAmer
SOMAscan
GOTERM
Graphpad Prism
SomaLogics
Excel
IPA
SOMAmers
Ingenuity Pathway Analysis ( IPA )

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