Zika virus impairs the development of blood vessels in a mouse model of congenital infection

Scientific Reports
Patricia P GarcezZ Molnár

Abstract

Zika virus (ZIKV) is associated with brain development abnormalities such as primary microcephaly, a severe reduction in brain growth. Here we demonstrated in vivo the impact of congenital ZIKV infection in blood vessel development, a crucial step in organogenesis. ZIKV was injected intravenously in the pregnant type 2 interferon (IFN)-deficient mouse at embryonic day (E) 12.5. The embryos were collected at E15.5 and postnatal day (P)2. Immunohistochemistry for cortical progenitors and neuronal markers at E15.5 showed the reduction of both populations as a result of ZIKV infection. Using confocal 3D imaging, we found that ZIKV infected brain sections displayed a reduction in the vasculature density and vessel branching compared to mocks at E15.5; altogether, cortical vessels presented a comparatively immature pattern in the infected tissue. These impaired vascular patterns were also apparent in the placenta and retina. Moreover, proteomic analysis has shown that angiogenesis proteins are deregulated in the infected brains compared to controls. At P2, the cortical size and brain weight were reduced in comparison to mock-infected animals. In sum, our results indicate that ZIKV impairs angiogenesis in addition to neurogenesis duri...Continue Reading

References

Jun 29, 1999·Molecular and Cellular Neurosciences·C LeventhalS A Goldman
Sep 7, 2000·The Journal of Comparative Neurology·T D PalmerF H Gage
Sep 18, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Holger GerhardtChrister Betsholtz
Nov 23, 2005·Oncology·Peter Carmeliet
Aug 6, 2008·Emerging Infectious Diseases·Robert S LanciottiMark R Duffy
Jan 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Richard DanemanBen A Barres
Apr 24, 2009·Cerebral Cortex·Daniel StubbsZoltán Molnár
Nov 19, 2013·Journal of Cellular and Molecular Medicine·Elizabeth A LogsdonFeilim Mac Gabhann
Feb 15, 2014·Clinics in Perinatology·Natalina SalmasoFlora M Vaccarino
Jun 4, 2014·American Journal of Physical Anthropology·Paula N GonzalezBenedikt Hallgrímsson
Sep 3, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Min ZouMengqing Xiang
Feb 11, 2016·The New England Journal of Medicine·Jernej MlakarTatjana Avšič Županc
Apr 4, 2016·Cell Stem Cell·Tomasz J NowakowskiArnold R Kriegstein
Apr 12, 2016·Science·Patricia P GarcezStevens K Rehen
Apr 14, 2016·Cell Host & Microbe·Helen M LazearMichael S Diamond
May 7, 2016·PLoS Neglected Tropical Diseases·Stuart D DowallRoger Hewson
May 24, 2016·PLoS Neglected Tropical Diseases·Matthew T AliotaJorge E Osorio
Jun 10, 2016·Nature·Fernanda R CugolaPatricia C B Beltrão-Braga
Aug 31, 2016·Nature Reviews. Microbiology·Carolyn B Coyne, Helen M Lazear
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hanna RetallackJoseph L DeRisi
Dec 8, 2016·Journal of Neurochemistry·Marta Machado-PereiraRaquel Ferreira
Dec 9, 2016·Neuron·Hongda LiJoseph G Gleeson
Jan 24, 2017·Scientific Reports·Patricia P GarcezStevens K Rehen
Mar 16, 2017·Development·Xuyu QianGuo-Li Ming
Jan 7, 2018·Science Immunology·Laura J YockeyAkiko Iwasaki

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Citations

Jan 27, 2019·The Journal of Comparative Neurology·Elizabeth B HutchinsonSharon L Juliano
Sep 29, 2019·Nature Communications·Nilda Vanesa Ayala-NunezRaphael Gaudin
Jul 10, 2019·Annual Review of Neuroscience·Kimberly M ChristianGuo-Li Ming
Apr 12, 2019·Frontiers in Cellular Neuroscience·Christian AlfanoLaurent Nguyen
Jul 25, 2019·Frontiers in Microbiology·Svetlana F KhaiboullinaSubhash C Verma
Feb 23, 2019·Journal of Anatomy·Javier Gilbert-JaramilloKieran Clarke
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Viviane Souza de CamposDaniel Adesse
Feb 9, 2020·The American Journal of Pathology·Gustavo GarciaVaithilingaraja Arumugaswami
May 27, 2021·Acta Neuropathologica Communications·Yi LiWenbo Zhang
Jun 8, 2021·Frontiers in Immunology·Cherley Borba Vieira AndradeTania Maria Ortiga-Carvalho
Aug 20, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Hannah E J YongPadma Murthi

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Methods Mentioned

BETA
PCR
fluorescence microscopy
Antibody Array

Software Mentioned

MOCK
Amira
ImageJ

Related Concepts