Zinc and copper play a role in coaggregation inhibiting action of Porphyromonas gingivalis

Oral Microbiology and Immunology
M Tamura, K Ochiai

Abstract

We investigated the mechanisms of adherence of salivary and serum proteins, which mimic gingival crevicular fluid (GCF), to Porphyromonas gingivalis, and the effects of these adhered proteins on coaggregation and hemagglutination properties. The amounts of salivary and serum proteins adhering to P. gingivalis were determined using (3)H-labeled and non-labeled proteins. The coaggregation between P. gingivalis and Streptococcus oralis or Streptococcus gordonii was observed. Hemagglutination was evaluated using sheep erythrocytes. Proteins that interacted with zinc or copper in saliva and serum and on P. gingivalis were examined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The amount of salivary or serum proteins that adhered to the surface of P. gingivalis strains was increased by cations, especially zinc and copper ions. The pretreatment of bacterial cells with salivary or serum proteins before the assay inhibited coaggregation with gram-positive bacteria and hemagglutination. These phenomena were enhanced by the presence of zinc or copper ions during the pretreatment of P. gingivalis with proteins. We detected protein bands that were related to these cations in saliva and serum and on P. gingivalis. Our find...Continue Reading

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Citations

Dec 19, 2012·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Toshiya Sato, Futoshi Nakazawa
Jun 14, 2012·International Journal of Dentistry·Masahiro YonedaTakao Hirofuji
Apr 8, 2014·Acta Biomaterialia·Ourania-Menti GoudouriAldo R Boccaccini
Sep 13, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alfonso Varela-LópezJosé L Quiles
Jun 16, 2018·Materials·Manuel Toledano-OsorioManuel Toledano
Feb 27, 2021·Journal of Dentistry·Manuel ToledanoRaquel Osorio
May 23, 2020·Acta Biomaterialia·Manuel Toledano-OsorioConrado Aparicio
Dec 7, 2018·Dental Materials : Official Publication of the Academy of Dental Materials·M C SánchezM Sanz

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