PMID: 2511329Nov 5, 1989Paper

Zinc-binding domain of poly(ADP-ribose)polymerase participates in the recognition of single strand breaks on DNA

Journal of Molecular Biology
J Ménissier-de MurciaGilbert de Murcia

Abstract

Poly(ADP-ribose)polymerase is a chromatin-associated enzyme of eukaryotic cell nuclei that catalyses the covalent attachment of ADP-ribose units from NAD+ to various nuclear acceptor proteins. This post-translational modification has been postulated to influence several chromatin functions, particularly those where nicking and rejoining of DNA occur. Poly(ADP-ribosyl)ation reactions are strictly dependent upon the presence of interruptions on DNA. We have recently demonstrated that the DNA-binding domain of the protein containing two putative "zinc-fingers" binds DNA in a zinc-dependent manner. The basis for the recognition of the DNA strand breaks by this enzyme, and more precisely, its 29,000 Mr N-terminal part, which contains the metal binding sites, needed to be clarified. DNA probes harbouring a single strand interruption at a defined position were constructed from synthetic oligonucleotides. DNase I protection studies show that poly(ADP-ribose)polymerase specifically binds to a DNA single-strand break by its metal-binding domain depending upon the presence of Zn(II). These results support the idea that the enzyme participates to the maintenance of DNA integrity in eukaryotes.

References

Jul 13, 1988·Biochimica Et Biophysica Acta·D LamarreG G Poirier
Jun 26, 1989·Nucleic Acids Research·A MazenG de Murcia
Aug 15, 1985·The Biochemical Journal·J C Gaal, C K Pearson
Oct 29, 1987·Biochemical and Biophysical Research Communications·K UchidaT Sugimura
Jul 1, 1988·Analytical Biochemistry·A MazenG de Murcia
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J Huet, A Sentenac
Jan 1, 1985·Annual Review of Biochemistry·K Ueda, O Hayaishi
Aug 1, 1984·European Journal of Biochemistry·P Zahradka, K Ebisuzaki

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Citations

Jan 1, 1993·Environmental and Molecular Mutagenesis·F R AlthausC Realini
Dec 15, 1994·Molecular & General Genetics : MGG·M A Collinge, F R Althaus
Sep 1, 1994·Molecular and Cellular Biochemistry·G de MurciaJ Ménissier de Murcia
Sep 1, 1994·Molecular and Cellular Biochemistry·R Alvarez-GonzalezH Mendoza-Alvarez
Sep 1, 1994·Molecular and Cellular Biochemistry·J LagueuxG G Poirier
Sep 1, 1994·Molecular and Cellular Biochemistry·F R AlthausC A Realini
May 26, 1993·Molecular and Cellular Biochemistry·D LautierG G Poirier
Oct 27, 1992·Biochimica Et Biophysica Acta·H ThomassinG G Poirier
Jan 1, 1995·Biochimie·M MassonC Niedergang
Apr 1, 1993·Toxicology Letters·T Boulikas
Sep 1, 1992·Mutation Research·A P EkerD Bootsma
Jan 30, 2013·Molecular Aspects of Medicine·Carles CantóPeter Bai
Dec 18, 2003·Neurobiology of Disease·Seong-Woon YuValina L Dawson
Jun 8, 2002·Archives of Biochemistry and Biophysics·John C Marecki, Joe M McCord
May 14, 1999·Trends in Pharmacological Sciences·A A PieperS H Snyder
Nov 6, 2003·Toxicology·Markus ChristmannBernd Kaina
Apr 24, 1999·Biochimie·F DantzerG de Murcia
Aug 24, 1999·The Biochemical Journal·D D'AmoursG G Poirier
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G GradwohlG de Murcia
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·F BunzB Stillman
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K UchidaM Miwa
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M de MurciaG de Murcia
Feb 2, 2008·The Journal of Biological Chemistry·Elizabeth Cotner-GoharaTom Ellenberger
Mar 3, 2011·Genes & Development·Sophie E Polo, Stephen P Jackson
Jul 15, 1997·European Journal of Biochemistry·S AoufouchiC C Ford
May 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Luis A EspinozaMark E Smulson
Sep 1, 2012·CNS Oncology·Ross Carruthers, Anthony J Chalmers

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