Abstract
Zinc is an essential cofactor required for the function of approximately 8% of the yeast and 10% of the human proteome. All of the "small Tim" proteins of the mitochondrial intermembrane space contain a strictly conserved "twin CX(3)C" zinc finger motif, which can bind zinc ions in the Cys-reduced form. We have shown previously that although disulfide bond formation is essential for the function of these proteins in mitochondria, only reduced proteins can be imported into mitochondria (Lu, H., Allen, S., Wardleworth, L., Savory, P., and Tokatlidis, K. (2004) J. Biol. Chem. 279, 18952-18958 and Morgan, B., and Lu, H. (2008) Biochem. J. 411, 115-122). However, the role of zinc during the import of these proteins is unclear. This study shows that the function of zinc is complex. It can play a thiol stabilizer role preventing oxidative folding of the small Tim proteins and maintaining the proteins in an import-competent form. On the other hand, zinc-bound forms cannot be imported into mitochondria efficiently. Furthermore, our results show that zinc is a powerful inhibitor of Erv1, an essential component of the import pathway used by the small Tim proteins. We propose that zinc plays a chaperone-like role in the cytosol during biog...Continue Reading
References
Jan 1, 1984·Endeavour·R J Williams
Feb 23, 1996·Science·J M Berg, Y Shi
Nov 15, 1996·Science·D Grady
Sep 8, 2000·The Journal of Biological Chemistry·U JakobJ C Bardwell
Dec 6, 2001·Biochemistry·W Tang, C C Wang
Mar 18, 2003·Inorganic Chemistry·Artur KrezelWojciech Bal
Mar 27, 2003·Traffic·Danielle LeuenbergerCarla M Koehler
Apr 1, 2003·IUBMB Life·Jacqueline M Matthews, Margaret Sunde
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stefano L SensiJohn H Weiss
Oct 8, 2003·Journal of Molecular Biology·Wei LiColin Kleanthous
Jan 20, 2004·Trends in Biochemical Sciences·Carla M Koehler
Feb 20, 2004·The Journal of Biological Chemistry·Hui LuKostas Tokatlidis
Feb 20, 2004·The Journal of Biological Chemistry·Hui LuKostas Tokatlidis
Mar 24, 2004·Biochemistry·Wolfgang Maret
Aug 6, 2004·The Journal of Biological Chemistry·Sean P CurranCarla M Koehler
Sep 11, 2004·The EMBO Journal·Agnieszka ChacinskaNikolaus Pfanner
Nov 4, 2004·Nature Structural & Molecular Biology·Nikolaus PfannerTrevor Lithgow
Dec 29, 2004·FEBS Letters·Nadia TerziyskaKai Hell
Jul 2, 2005·Cell·Kostas Tokatlidis
Aug 25, 2005·Methods in Molecular Biology·Chris MeisingerKaye N Truscott
Oct 4, 2005·Journal of Molecular Biology·Hui Lu, Joanna Woodburn
Oct 26, 2005·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Wolfgang Maret
May 6, 2006·Biochimica Et Biophysica Acta·David J Eide
Feb 15, 2007·Biochemistry·Wenzhong WangColin Thorpe
May 29, 2007·Eukaryotic Cell·Claudia SimmDavid J Eide
Oct 30, 2007·Proteins·Ekaterina IvanovaHui Lu
Nov 6, 2007·Molecular Biology of the Cell·Judith M MüllerAgnieszka Chacinska
Nov 21, 2007·Journal of Molecular Biology·Ekaterina IvanovaHui Lu
Dec 14, 2007·The Biochemical Journal·Bruce Morgan, Hui Lu
Jan 9, 2008·Biochimica Et Biophysica Acta·Kai Hell
Sep 13, 2008·EMBO Reports·Nikola MeseckeJohannes M Herrmann
Oct 25, 2008·Nature·Steve TotteyNigel J Robinson
Citations
Jan 1, 1992·Life Sciences·T IkedaK Takatsuki
Feb 19, 2014·European Journal of Medicinal Chemistry·Huanhuan QuYongmin Zhang
Aug 15, 2009·The Journal of Biological Chemistry·Swee Kim Ang, Hui Lu
Dec 14, 2011·The Journal of Biological Chemistry·Johannes M Herrmann, Jan Riemer
Apr 7, 2010·Antioxidants & Redox Signaling·Johannes M Herrmann, Jan Riemer
Feb 9, 2010·Antioxidants & Redox Signaling·Toshiya EndoShin Kawano
Mar 11, 2010·Antioxidants & Redox Signaling·Dionisia P Sideris, Kostas Tokatlidis
Oct 19, 2012·Antioxidants & Redox Signaling·Péter Nagy
Jul 30, 2010·Human Molecular Genetics·Helge UhrigshardtTracey A Rouault
Sep 2, 2009·Journal of Biochemistry·Marcel Deponte, Kai Hell
Aug 16, 2013·International Journal of Molecular Sciences·Efrain Ceh-PaviaHui Lu
Jan 12, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·David B BriggsTsu-Shuen Tsao
Apr 15, 2011·Trends in Plant Science·Cécile NouetMarc Hanikenne
Mar 1, 2011·The FEBS Journal·Jonathan Martínez-FábregasMiguel Á De la Rosa
Aug 11, 2012·EMBO Reports·Romina DurigonHui Lu
Jun 12, 2014·Protein Science : a Publication of the Protein Society·Clara IannuzziAnnalisa Pastore
Oct 29, 2009·The FEBS Journal·Adam M Benham
Dec 19, 2013·International Journal of Cell Biology·Manuel Fischer, Jan Riemer
Jul 31, 2012·Journal of Structural Biology·Lucia BanciJulia Winkelmann
Aug 26, 2009·Cell·Agnieszka ChacinskaNikolaus Pfanner
Jul 20, 2016·Biochemistry·Paul A Lindahl, Michael J Moore
Aug 21, 2016·Cell and Tissue Research·Alican J Erdogan, Jan Riemer
Jun 28, 2016·ELife·Valentina PelehJohannes M Herrmann
Jul 10, 2013·The Biochemical Journal·Michael P SpillerHui Lu
Jul 17, 2015·Bioscience Reports·Michael P SpillerHui Lu
May 11, 2018·Oxidative Medicine and Cellular Longevity·Sung Ryul Lee
Nov 30, 2020·FEBS Letters·Lena KrämerJohannes M Herrmann
Jan 19, 2021·Journal of Food Biochemistry·Ademola Adetokunbo OyagbemiMomoh Audu Yakubu
Sep 5, 2009·Chemical Reviews·Wolfgang Maret, Yuan Li