Zinc chelation enhances thyroid hormone induction of growth hormone mRNA in GH3 cells

Molecular and Cellular Endocrinology
S Chattopadhyay, Hedley C Freake

Abstract

The effects of restriction and addition of zinc on thyroid hormone responsiveness of the growth hormone gene were investigated in GH3, rat pituitary tumor cells. Addition of diethylenetriaminepenta-acetic acid (DTPA), a membrane-impermeable chelator, resulted in up to 10-fold increases in GH mRNA in the presence of 10 nM T3, with half-maximal induction at 50 microM DTPA. Only minor effects were seen in the absence of T3. Addition of zinc inhibited the stimulatory effect of DTPA in a dose-dependent manner. Equimolar concentrations of other divalent cations could not substitute for zinc, though inhibitions of the DTPA effect were observed at higher concentrations. In the absence of DTPA, exogenous zinc (100 microM) inhibited T3-induced GH mRNA by approximately 33%. Addition of DTPA or zinc did not affect T3 binding to its nuclear receptor. DTPA also enhanced the stimulatory effect of dexamethasone on GH mRNA. The results demonstrate that restricted zinc availability positively affects T3 induction of the GH gene in GH3 cells.

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Citations

Jun 26, 2003·Bioorganic & Medicinal Chemistry Letters·Nathaniel C LimChristian Brückner
Feb 10, 2016·Environmental Science and Pollution Research International·Jian LiNa Li
Mar 10, 2009·The Journal of Nutritional Biochemistry·Amrita DuttaHedley C Freake
Apr 4, 2001·The Journal of Nutrition·H C FreakeS A Zinn

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