Zinc-finger protein 545 is a novel tumour suppressor that acts by inhibiting ribosomal RNA transcription in gastric cancer

Gut
Shiyan WangJun Yu

Abstract

Zinc-finger protein 545 (ZNF545) is a member of the family of Krüppel-associated box-containing zinc-finger proteins. The aim of this study was to clarify its biological function as a tumour suppressor in gastric cancer. The biological function of ZNF545 was determined by cell growth and apoptosis assays. The ZNF545 target signal pathway was identified by promoter luciferase assay, northern blot, run-on transcription assay, chromatin immunoprecipitation and coimmunoprecipitation assays. The clinical application of ZNF545 was assessed in primary gastric cancers. ZNF545 was silenced or reduced in 16 out of 18 gastric cancer cell lines by promoter hypermethylation. Restoration of ZNF545 expression in gastric cancer cell lines suppressed cell proliferation and induced apoptosis. These effects of ZNF545 were attributed to inhibition of ribosomal RNA (rRNA) transcription. Inhibition of rRNA transcription by ZNF545 was further revealed to be associated with direct ribosomal DNA (rDNA) promoter binding, recruitment of the corepressor, heterochromatin protein 1β, and reduction of trimethylated histone H3 at the Lys4 residue at the rDNA locus. ZNF545 methylation was detected in 51.9% (41/79) of gastric cancer tissues, 27.0% (20/74) of ad...Continue Reading

Citations

Nov 17, 2012·Biochimica Et Biophysica Acta·K M HannanR B Pearson
Sep 18, 2013·Helicobacter·Ceu FigueiredoJose C Machado
Jul 26, 2014·Current Opinion in Plant Biology·Xi HuangXing Wang Deng
Mar 30, 2017·Expert Review of Gastroenterology & Hepatology·Yongzhan NieDaiming Fan
Jun 3, 2016·Microbiology and Molecular Biology Reviews : MMBR·Rakesh SrivastavaSeong Hoon Ahn
Oct 17, 2014·World Journal of Gastroenterology : WJG·Jing ShiPeng Hou
May 23, 2017·Oncology Letters·Fuminori SonoharaShuji Nomoto
Jan 16, 2014·Molecular Cancer Research : MCR·Changwen ZhangSijin Liu
Feb 13, 2021·International Journal of Genomics·Fengqing ShaoWencai Wang
Mar 7, 2021·International Journal of Molecular Sciences·Joanna SobocińskaUrszula Oleksiewicz

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