Zinc finger protein, Hzf, is required for megakaryocyte development and hemostasis

The Journal of Experimental Medicine
Yuki KimuraAlan Bernstein

Abstract

Using an expression gene trapping strategy, we recently identified a novel gene, hematopoietic zinc finger (Hzf), which encodes a protein containing three C(2)H(2)-type zinc fingers that is predominantly expressed in megakaryocytes. Here, we have examined the in vivo function of Hzf by gene targeting and demonstrated that Hzf is essential for megakaryopoiesis and hemostasis in vivo. Hzf-deficient mice exhibited a pronounced tendency to rebleed and had reduced alpha-granule substances in both megakaryocytes and platelets. These mice also had large, faintly stained platelets, whereas the numbers of both megakaryocytes and platelets were normal. These results indicate that Hzf plays important roles in regulating the synthesis of alpha-granule substances and/or their packing into alpha-granules during the process of megakaryopoiesis.

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Citations

Oct 31, 2007·Biological Trace Element Research·Sławomir TubekIwona Tubek
Mar 5, 2010·Hybridoma·Hideaki NakamuraMasataka Sugimoto
Aug 13, 2008·Current Opinion in Hematology·Qiansheng RenSidney W Whiteheart
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Jul 19, 2013·The Journal of Clinical Investigation·Carsten DeppermannBernhard Nieswandt
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Takatoshi IijimaHideyuki Okano
Apr 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yuki KimuraAlan Bernstein
Jan 9, 2008·Blood Reviews·Isabelle I SallesHans Deckmyn
Mar 27, 2007·Neuroscience Research·Takatoshi IijimaHideyuki Okano
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Mar 10, 2017·Platelets·Chang Hua ChenWalter H A Kahr

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Methods Mentioned

BETA
PCR
Assay
electrophoresis
genotyping
targeted mutation
transmission electron microscopy
gene trapping
targeted mutations

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