Zinc increases EGF-stimulated DNA synthesis in primary mouse hepatocytes. Studies in tumor promoter-treated cell cultures

Biochemical Pharmacology
A B Kobusch, K W Bock

Abstract

To investigate factors influencing cell proliferation, cells are often cultured in serum-free medium. In the present study it is shown that addition of zinc chloride (40 microM) to primary mouse hepatocytes, cultured in Dulbecco's minimal essential medium, markedly enhanced growth factor (EGF)-stimulated [3H]thymidine incorporation into DNA. Treatment of cell cultures with phenobarbital or 3,4,3',4'-tetrachlorobiphenyl (enzyme inducers and tumor promoters in vivo) or with 12-O-tetradecanoylphorbol-13-acetate (the classical skin tumor promoter) further increased EGF-stimulated DNA synthesis. The results emphasize the need to adequately substitute zinc in serum-free cultured cells.

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Jan 1, 1993·Cell Biology and Toxicology·D WölfleC Schmutte
Oct 1, 1995·Journal of the American College of Nutrition·M K SongM E Ament
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Dec 1, 1994·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·P TarnowJ O Jansson

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