Zinc inhibition of adenylyl cyclase correlates with conformational changes in the enzyme

Cellular Signalling
Claudette KleinR K Sunahara

Abstract

We have previously demonstrated that Zn(2+) inhibits hormone and forskolin stimulation of cAMP synthesis in intact N18TG2 cells, corresponding plasma membranes, and of recombinant adenylyl cyclase isoforms. If, however, the enzyme is pre-activated by hormone or forskolin, Zn(2+) inhibition is attenuated [J. Biol. Chem. 277 (2002) 11859]. We have extended our analyses of this inhibition to investigations of soluble adenylyl cyclase, composed of the CI and CII domains of the full-length protein. The properties of Zn(2+) inhibition of the soluble enzyme parallel that of the full-length protein, including the fact that inhibition is not competitive with Mg(2+). By monitoring intrinsic and extrinsic fluorescence, we demonstrate changes in enzyme conformers in response to the addition of varied effectors. The data suggest a possible mechanism by which Zn(2+) inhibits adenylyl cyclase activity.

References

Mar 20, 1997·Nature·G ZhangJ H Hurley
Aug 29, 1997·The Journal of Biological Chemistry·R K SunaharaA G Gilman
Jan 1, 1997·Neurobiology of Disease·M P Cuajungco, G J Lees
Jul 25, 1998·The Journal of Biological Chemistry·G ZimmermannR Taussig
Jan 23, 1999·Current Opinion in Structural Biology·J J Tesmer, S R Sprang
Jul 31, 1999·Science·J J TesmerS R Sprang
May 10, 2000·The Journal of Nutrition·A Q Truong-TranP D Zalewski
May 10, 2000·The Journal of Nutrition·C J FredericksonR B Thompson
Jan 24, 2002·The Journal of Biological Chemistry·Claudette KleinAllyn C Howlett
May 6, 2003·The Journal of Nutrition·Klaus-Helge Ibs, Lothar Rink

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Citations

Sep 22, 2006·Antioxidants & Redox Signaling·Irina Korichneva
Apr 30, 2009·Annual Review of Nutrition·Hajo Haase, Lothar Rink
Jan 31, 2012·Journal of Toxicology·Sanah SadiqDietrich Büsselberg
Apr 25, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hajo Haase, Lothar Rink
Jul 23, 2009·Synapse·Byron K Y Bitanihirwe, Miles G Cunningham
Jul 12, 2005·Biochemical and Biophysical Research Communications·Seiji YamashitaSigeo Ihara
Aug 3, 2010·Neurochemistry International·Masaki AndoAtsushi Takeda
Dec 4, 2014·International Journal of Medical Microbiology : IJMM·Wesam SalemStefan Schild
Sep 22, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Samantha HagertyVitaly Vodyanoy
Jan 5, 2016·Metallomics : Integrated Biometal Science·K A Taylor, N Pugh
Oct 25, 2017·International Journal of Molecular Sciences·Martina MaywaldLothar Rink
Dec 1, 2017·Nutrients·Inga WesselsLothar Rink
Jan 22, 2005·American Journal of Physiology. Cell Physiology·Weidong GengOrson W Moe
May 28, 2020·Biological Trace Element Research·Mohammad Tariqur Rahman, Syed Zahir Idid
Dec 23, 2021·Proceedings of the National Academy of Sciences of the United States of America·Brian S MunteanKirill A Martemyanov

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