Zinc ions block H⁺/OH⁻ channels in Chara australis

Plant, Cell & Environment
Sabah Al Khazaaly, M J Beilby

Abstract

Chara australis cells exposed to media of pH 10 and above exhibit high conductance, arising from the opening of H⁺/OH⁻ channels in the plasma membrane. This high conductance can be totally inhibited by 1.0 mm ZnCl₂ and restored by 0.5 mm 2-mercaptoethanol (ME). Important for carbon fixation, H⁺/OH⁻ channels play a key role in cell pH banding. Banding was also shown to be abolished by 1.0 mm ZnCl₂ and restored in some cells by ME. The proton pump is also involved in banding, but was little affected by ZnCl₂ over the periods needed for the inhibition of H⁺/OH⁻ channels. Previously, we postulated that H⁺/OH⁻ channels open transiently at the onset of saline stress in salt-sensitive C. australis, causing membrane potential difference (PD) noise; and remain open in latter stages of saline stress, contributing to cell deterioration. ZnCl₂ totally inhibited the saline noise and the upwardly concave I/V characteristics associated with the putative H⁺/OH⁻ currents. Again, ME reversed both these effects. We discuss the mode of action of zinc ions and ME with reference to animal voltage-gated H⁺ channels and water channels.

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Citations

Dec 20, 2013·Journal of the Royal Society, Interface·Thomas E DeCoursey, Jonathan Hosler
Feb 15, 2016·Journal of Plant Research·Vilma KisnierieneOsvaldas Ruksenas
Nov 8, 2014·The Journal of Membrane Biology·Mary J BeilbyMary A Bisson
Jul 16, 2013·Biochimica Et Biophysica Acta·Alexander A BulychevTatiana N Bibikova
Jul 24, 2012·Trends in Plant Science·Alison R TaylorGlen L Wheeler
Sep 30, 2019·Journal of Experimental Botany·Anna AlovaAlexander Bulychev
Nov 28, 2019·American Journal of Physiology. Cell Physiology·Liyo KaoIra Kurtz

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