Zinc ions inhibit oxidation of cytochrome c oxidase by oxygen

FEBS Letters
Anna Aagaard, Peter Brzezinski

Abstract

Cytochrome c oxidase is a membrane-bound enzyme that catalyses the reduction of O2 to H2O and uses part of the energy released in this reaction to pump protons across the membrane. We have investigated the effect of addition of Zn2+ on the kinetics of two reaction steps in cytochrome c oxidase that are associated with proton pumping; the peroxy to oxo-ferryl (P(r)-->F) and the oxo-ferryl to oxidised (F-->O) transitions. The Zn2+ binding resulted in a decrease of the F-->O rate from 820 s(-1) (no Zn2+) to a saturating value of approximately 360 s(-1) with an apparent K(D) of approximately 2.6 microM. The P(r)-->F rate (approximately 10[(4) s(-1)] before addition of Zn2+) decreased more slowly with increasing Zn2+ concentration and a K(D) of approximately 120 microM was observed. The effects on both kinetic phases were fully reversible upon addition of EDTA. Since both the P(r)-->F and F-->O transitions are associated with proton uptake through the D-pathway, a Zn2+-binding site is likely to be located at the entry point of this pathway, where several carboxylates and histidine residues are found that may co-ordinate Zn2+.

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Citations

Jul 17, 2012·Chemical Reviews·Kasper P Kepp
Sep 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Naoya TeraharaMasahiro Ito
May 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ann-Louise JohanssonPeter Brzezinski
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kazumasa MuramotoShinya Yoshikawa
Feb 3, 2007·FEBS Letters·Francesco FranciaSergio Papa
Aug 29, 2006·Biochimica Et Biophysica Acta·Colin A Wraight
Apr 21, 2006·Biochimica Et Biophysica Acta·Armen Y MulkidjanianDmitry A Cherepanov
Aug 11, 2007·Biochimica Et Biophysica Acta·Mårten Wikström, Michael I Verkhovsky
Apr 22, 2004·Biochimica Et Biophysica Acta·Pia Adelroth, Peter Brzezinski
Jun 30, 2006·Biochimica Et Biophysica Acta·Kristina FaxénPeter Brzezinski
Feb 27, 2010·Biochimica Et Biophysica Acta·Peter Brzezinski, Ann-Louise Johansson
Sep 23, 2014·Biochemistry·Marius SchulteThorsten Friedrich
Sep 30, 2016·Scientific Reports·Simone GrafPeter Brzezinski
Oct 22, 2016·Chemistry : a European Journal·Joanna WątłyMagdalena Rowińska-Żyrek
Sep 19, 2006·The Journal of Biological Chemistry·Mark S Sharpley, Judy Hirst
Feb 8, 2002·The Journal of Biological Chemistry·Denise A MillsShelagh Ferguson-Miller
Nov 15, 2019·FEBS Letters·Jóhanna VilhjálmsdóttirPeter Brzezinski
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Dec 29, 2019·Scientific Reports·Markus L BjörckPeter Brzezinski
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Mar 24, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Alexei StuchebrukhovPeter Brzezinski
Aug 22, 2020·Chemical Reviews·Frederic Melin, Petra Hellwig
Oct 23, 2008·The Journal of Physical Chemistry. B·Haiyu WangNeal W Woodbury
Feb 16, 2006·Biochemistry·Hiroshi IshikitaErnst-Walter Knapp
Jun 30, 2021·Chemical Reviews·Peter BrzezinskiPia Ädelroth

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