Zinc is an essential cofactor for type I isopentenyl diphosphate:dimethylallyl diphosphate isomerase

Journal of the American Chemical Society
Christina N Carrigan, C Dale Poulter

Abstract

Isopentenyl diphosphate (IPP) isomerase catalyzes the interconversion of IPP and dimethylallyl diphosphate (DMAPP). This is an essential reaction in the mevalonate pathway for biosynthesis of isoprenoid compounds. A crystal structure of Escherichia coli type I IPP isomerase shows a his3glu2 octahedral metal binding site (Durbecq, V. et al. EMBO, 2001, 20, 1530-1537). A metal ion analysis of recombinant E. coli type I IPP isomerase purified from metal-free buffer or buffer containing 10 muM ZnCl2 and 10 muM MnCl2 indicated that the protein contained one atom of Zn2+ per molecule. The metal content and the activity of the enzyme did not change when dialyzed for 6 h against metal-free buffer but rapidly decreased upon dialysis against buffer containing o-phenanthroline. Structural and catalytic roles for Zn2+ are discussed.

References

Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·J B BonannoS K Burley

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Citations

Nov 2, 2013·Chemical Reviews·Alexander R MoiseEleanore T Wurtzel
Jun 6, 2007·Journal of the American Chemical Society·Jonathan B JohnstonC Dale Poulter
Apr 5, 2012·Journal of the American Chemical Society·Venkatadurga JonnalagaddaJohn P Richard
Apr 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jérôme de RuyckJohan Wouters
Dec 4, 2012·International Journal of Molecular Sciences·Pablo Sobrado
Sep 12, 2014·FEMS Microbiology Reviews·Kyle W Becker, Eric P Skaar
Dec 2, 2006·Journal of Molecular Biology·Cheng ZhangWeimin Gong
Jan 5, 2007·The Journal of Biological Chemistry·Daun B ClizbeSkaidrite K Krisans
Nov 5, 2005·Biochemical and Biophysical Research Communications·Jérôme de RuyckJohan Wouters
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara
Aug 31, 2006·Journal of the American Chemical Society·Sungwon Lee, C Dale Poulter

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