Zinc L-Carnosine Protects CCD-18co Cells from L-Buthionine Sulfoximine-Induced Oxidative Stress via the Induction of Metallothionein and Superoxide Dismutase 1 Expression.

Biological Trace Element Research
Theng Choon OoiRazinah Sharif

Abstract

Zinc L-carnosine (ZnC) is the chelate form of zinc and L-carnosine and is one of the zinc supplements available in the market. This study aims to determine the protective effects of ZnC against L-buthionine sulfoximine (BSO)-induced oxidative stress in CCD-18co human normal colon fibroblast cell line. CCD-18co cells were pretreated with ZnC (0-100 μM) for 24 h before the induction of oxidative stress by BSO (1 mM) for another 24 h. Results from this present study demonstrated that ZnC up to the concentration of 100 μM was not cytotoxic to CCD-18co cells. Induction with BSO significantly increased the intracellular reactive oxygen species (ROS) levels and reduced the intracellular glutathione (GSH) levels in CCD-18co cells. Pretreatment with ZnC was able to attenuate the increment in intracellular ROS level in CCD-18co cells significantly in a concentration-dependent manner. However, ZnC did not have any effects on intracellular GSH levels and Nrf2 activation. Mechanistically, pretreatment with ZnC was able to upregulate the expression of metallothionein (MT) and superoxide dismutase 1 (SOD1) in CCD-18co cells. Results from dual-luciferase reporter gene assay reported that ZnC was able to increase the MRE-mediated relative lucif...Continue Reading

References

Mar 1, 1993·Free Radical Biology & Medicine·M Sato, I Bremner
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·G K Andrews
Sep 10, 2002·Parkinsonism & Related Disorders·Catherine MytilineouJocelyn A Yabut
Jan 30, 2004·The Journal of Toxicological Sciences·Toshiyuki WatanabeSunao Manabe
Nov 16, 2004·Mechanisms of Ageing and Development·Alex BokovArlan Richardson
Apr 26, 2005·Inorganic Chemistry·Vladimir Pelmenschikov, Per E M Siegbahn
Mar 22, 2008·Free Radical Biology & Medicine·Miriam M CorteseVictoria Kolb-Bachofen
Jul 8, 2008·Molecular Aspects of Medicine·Shelly C Lu
Sep 18, 2008·Molecular Aspects of Medicine·Henry Jay FormanAlessandra Rinna
Dec 9, 2008·Breast Cancer Research : BCR·Joan S Lewis-WambiV Craig Jordan
Jan 15, 2010·Methods in Molecular Biology·Evgeniy Eruslanov, Sergei Kusmartsev
Jan 11, 2011·Mutation Research·Thomas B KrystonAlexandros G Georgakilas
Jan 12, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wolfgang Maret
Feb 1, 2012·Biochimica Et Biophysica Acta·Viola GüntherWalter Schaffner
May 9, 2012·Free Radical Research·Abigail F Smith, George Loo
Sep 22, 2012·Biochimica Et Biophysica Acta·Shelly C Lu
Feb 26, 2013·Free Radical Biology & Medicine·Suryakant K NitureAnil K Jaiswal
Mar 19, 2013·International Journal of Molecular Sciences·Branislav Ruttkay-NedeckyRene Kizek
Oct 19, 2013·Physiological Reviews·Alexander A BoldyrevWim Derave
Sep 11, 2014·Frontiers in Pharmacology·Katia AquilanoMaria R Ciriolo
Nov 18, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Long HeXi Ma
Apr 28, 2018·Circulation Research·Steven J ForresterKathy K Griendling

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
transfection

Software Mentioned

Statistical Package for Social Sciences ( SPSS )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.