Zinc modulation of GABAA receptor-mediated chloride flux in rat hippocampal slices

Brain Research
M GordeyI Spigelman

Abstract

We studied the effect of ZnCl2 application on GABAA receptor-mediated 36CI- flux in microsacs prepared from whole rat hippocampus and in region-specific hippocampal slices. Slices were obtained from the dentate gyrus (DG), which contains the zinc-enriched hilar region, and from the CA1 region which contains lower levels of endogenous zinc. Muscimol (10 microM)-evoked 36Cl- flux was significantly reduced by ZnCl2 (100 microM) in hippocampal microsacs. In hippocampal slices, muscimol (50 microM)-evoked 36Cl- efflux was higher in CA1 (112.5 +/- 27.9% above basal efflux rate) than in DG slices (29.7 +/- 5.6%). In the presence of ZnCl2, the muscimol effect on efflux rate in CA1 and DG regions was decreased to 10.6 +/- 5.4% and 6.9 +/- 4.9%, respectively. Preincubation with the zinc chelator, tetrakis(2-pyridylmethyl)ethylenediamine (TPEN, 20 microM), caused a significant increase in muscimol-evoked 36Cl- efflux only in DG slices (57.2 +/- 7.0%), suggesting that GABAA receptors in the DG of rat hippocampus under physiological conditions may function under the inhibitory influence of endogenous chelatable zinc. In intracellular recordings, ZnCl2 (100 microM) application had no effect on the responses to GABA applied perisomatically or...Continue Reading

References

Sep 30, 1983·Neuroscience Letters·T G Smart, A Constanti
Apr 19, 1984·Nature·S Y Assaf, S H Chung
Apr 19, 1984·Nature·G A HowellC J Frederickson

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Citations

Jan 14, 1998·Brain Research. Developmental Brain Research·J V Sanchez-AndresC Lopez-Gracia
Jun 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·D J Rowe, D J Bobilya
Mar 6, 2002·Microscopy Research and Technique·Carlos López-GarcíaAsunción Molowny
Jan 19, 2021·Neuroscience·Rebecca F KrallElias Aizenman

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