Zinc porphyrin as a donor for FRET in Zn(II)cytochrome c

Journal of the American Chemical Society
Andrea J LeeKara L Bren

Abstract

We demonstrate that Zn(II) porphyrin in Zn(II)cytochrome c (Zn cyt c) is a fluorescence resonance energy transfer (FRET) donor to an Alexa660 dye acceptor. The energy transfer efficiency is dependent on the distance between the two fluorophores as shown through protein denaturation studies of five Zn cyt c variants labeled with Alexa660 in different positions. The relative quantum yield, excitation and emission energies, and labeling efficiencies of this donor-acceptor pair allow for a method of analysis based on sensitized emission of the acceptor. These studies show that Zn(II) porphyrin is an effective energy donor for measurement of molecular-scale distances by FRET.

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Citations

Oct 12, 2012·Applied Microbiology and Biotechnology·Sohila ZadranMichel Baudry
Oct 17, 2012·Journal of the American Chemical Society·Yuning HongEkaterina V Pletneva
May 27, 2011·Chemical Communications : Chem Comm·Mei-Sheng WuHong-Yuan Chen
Dec 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jonas HanskeEkaterina V Pletneva
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chris L BergstromJohn T Groves
Dec 17, 2015·Inorganic Chemistry·Banu KandemirKara L Bren
Oct 17, 2015·Journal of the American Chemical Society·Matthias Klaper, Torsten Linker
Aug 28, 2015·Journal of the American Chemical Society·Vitaly V Chaban, Oleg V Prezhdo
Feb 12, 2014·Metallomics : Integrated Biometal Science·Antonio RanieriMarco Sola
Sep 4, 2014·Chemical Communications : Chem Comm·Selen ManiogluHalil Bayraktar
Jul 16, 2011·Angewandte Chemie·Long YiYao-Wen Wu
Oct 27, 2017·Physical Chemistry Chemical Physics : PCCP·Tsuyoshi MashimaTakashi Hayashi
Apr 14, 2021·Journal of the American Chemical Society·Edward R H WalterNicholas J Long
Feb 5, 2013·The Journal of Physical Chemistry. a·Davis B Moravec, Michael D Hopkins
Jun 18, 2015·Accounts of Chemical Research·Jesse G Kleingardner, Kara L Bren
Oct 5, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Edward R H WalterNicholas J Long

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