Zinc Promotes Adipose-Derived Mesenchymal Stem Cell Proliferation and Differentiation towards a Neuronal Fate

Stem Cells International
Mi-Young MoonSang Won Suh

Abstract

Zinc is an essential element required for cell division, migration, and proliferation. Under zinc-deficient conditions, proliferation and differentiation of neural progenitors are significantly impaired. Adipose-derived mesenchymal stem cells (AD-MSCs) are multipotent stem cells that can differentiate into neurons. The aim of this study was to evaluate the effect of zinc on AD-MSC proliferation and differentiation. We initially examined the effect of zinc on stem cell proliferation at the undifferentiated stage. AD-MSCs showed high proliferation rates on day 6 in 30 μM and 100 μM of ZnCl2. Zinc chelation inhibited AD-MSC proliferation via downregulation of ERK1/2 activity. We then assessed whether zinc was involved in cell migration and neurite outgrowth during differentiation. After three days of neuronal differentiation, TUJ-1-positive cells were observed, implying that AD-MSCs had differentiated into early neuron or neuron-like cells. Neurite outgrowth was increased in the zinc-treated group, while the CaEDTA-treated group showed diminished, shrunken neurites. Furthermore, we showed that zinc promoted neurite outgrowth via the inactivation of RhoA and led to the induction of neuronal gene expression (MAP2 and nestin) in diff...Continue Reading

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Citations

Aug 9, 2019·International Journal of Molecular Sciences·Paulina KazimierczakAgata Przekora
Oct 14, 2020·Journal of Molecular Neuroscience : MN·Princy ChoudharySangeeta Singh
Dec 2, 2020·International Journal of Molecular Sciences·Seunghyuk ChoiSang Won Suh
Jan 6, 2021·European Journal of Nutrition·Vijay KumarJong-Joo Kim
Oct 31, 2020·Biofabrication·Zahra Sadat GhazaliThomas Braschler
Dec 16, 2020·ACS Biomaterials Science & Engineering·Vianni ChopraDeepa Ghosh

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Methods Mentioned

BETA
electrophoresis
flow cytometry
pull-down
FACS

Software Mentioned

ImageJ
GraphPad Prism
Zen microscopy
GraphPad
WinMDI

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