Zinc transporter 3 immunohistochemical tracing of sprouting mossy fibres

Neurochemistry International
Zhi-Hong ChiZhan-You Wang

Abstract

Zinc transporter 3 (ZNT3) has been shown to transport zinc ions from the cytosol into presynaptic vesicles in the mammalian brain. Several studies have stated that the zinc ion containing synaptic vesicles of zinc-enriched neurons (ZEN) are loaded with ZNT3 proteins in their membranes. This fact makes it possible to trace sprouting mossy fibres in the temporal lobe epileptic hippocampus. In the present study, we examined the expression and distribution patterns of ZNT3 protein and chelatable zinc ions in the mouse hippocampus after pilocarpine treatment. Our results demonstrate that both ZNT3 immunostaining and autometallography reveal identical patterns of sprouting mossy fibres in the inner molecular layer in the mouse hippocampus. Using ZNT3 immuno-electron microscopic analysis we confirmed the presence of ectopic mossy fibre terminals in the inner molecular layer and found additionally by immuno-blotting a significant increase of ZNT3 in the pilocarpine-treated mouse hippocampi compared to age-matched controls. The increase of ZNT3 after pilocarpine treatment was time-dependent. The results support the notion that ZNT3 immunohistochemistry provides an excellent tool for tracing sprouting of ZEN terminals. The progressive in...Continue Reading

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Citations

Nov 3, 2010·Learning & Memory·Guillaume MartelGleb P Shumyatsky
Jan 24, 2012·Experimental Neurology·Xiao-Xin YanPeter R Patrylo
Feb 5, 2014·Neurobiology of Disease·Fredrik BerglindMerab Kokaia
Aug 16, 2017·Biological Trace Element Research·Abdulkerim Kasim BaltaciRasim Mogulkoc
Jul 18, 2019·Frontiers in Neurology·Dominik KobylarekWojciech Kozubski
Apr 19, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Joanna WojtkiewiczSławomir Gonkowski
Nov 26, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Toshikazu KakizakiYuchio Yanagawa
Sep 29, 2009·Neurochemistry International·Sanja Pavlica, Rolf Gebhardt
Dec 22, 2021·Developmental Neurobiology·Yurika NakanishiToshio Ohshima

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