ZipN is an essential FtsZ membrane tether and contributes to the septal localization of SepJ in the filamentous cyanobacterium Anabaena

Scientific Reports
Sergio CamargoAntonia Herrero

Abstract

The organismic unit of heterocyst-forming cyanobacteria is a filament of communicating cells connected by septal junctions, proteinaceous structures bridging the cytoplasms of contiguous cells. This distinct bacterial organization is preserved during cell division. In Anabaena, deletion of the zipN gene could not be segregated. We generated strain CSL109 that expresses zipN from a synthetic regulatable promoter. Under conditions of ZipN depletion, cells progressively enlarged, reflecting restricted cell division, and showed drastic morphological alterations including cell detachment from the filaments, to finish lysing. In contrast to the wild-type localization in midcell Z-rings, FtsZ was found in delocalized aggregates in strain CSL109. Consistently, the proportion of membrane-associated to soluble FtsZ in fractionated cell extracts was lower in CSL109. Bacterial two-hybrid analysis showed that ZipN interacts with FtsZ and other cell-division proteins including cytoplasmic Ftn6 and SepF, and polytopic FtsW, FtsX, FtsQ and FtsI. Additionally, ZipN interacted with the septal protein SepJ, and in CSL109 depletion of ZipN was concomitant with a progressive loss of septal specificity of SepJ. Thus, in Anabaena ZipN represents an e...Continue Reading

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Citations

Feb 11, 2020·Molecular Microbiology·Benjamin L SpringsteinTal Dagan
Oct 29, 2020·FEBS Open Bio·Benjamin L SpringsteinKarina Stucken
Sep 10, 2020·Journal of Bacteriology·Maria L White, Prahathees J Eswara
Dec 23, 2020·Life·Benjamin L SpringsteinKarina Stucken
Mar 7, 2021·Current Opinion in Microbiology·Ann-Katrin Kieninger, Iris Maldener

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Methods Mentioned

BETA
PCR
fluorescence microscopy
confocal microscopy
two hybrid
electrophoresis
Fluorescence
light-microscopy

Software Mentioned

Image Lab
constrained
BACTH
MEMSAT
SOSUI
Leica Application Suite Advanced Fluorescence
TMHMM
ImageJ
RStudio Desktop
TMpred

Related Concepts