ZNF32 inhibits autophagy through the mTOR pathway and protects MCF-7 cells from stimulus-induced cell death

Scientific Reports
Yanyan LiYuquan Wei

Abstract

ZNF32 is a recently identified zinc finger protein and its functions remain largely unknown. Autophagy has been shown to affect cell proliferation and survival. Here, we innovatively show the effect of ZNF32 on cell autophagy and autophagy-associated cell death in breast carcinoma cells and also elucidate its underlying mechanisms. We examined the autophagic activity and LC3 II expression in human carcinoma cell lines with increased or decreased ZNF32 expression. Pharmacological inhibition (rapamycin) or activation (EGF) assays were used to investigate the function of the AKT/mTOR pathway during this process. H2O2- and diamide-induced MCF-7 cell death models were used to elucidate the role of ZNF32-associated autophagy in breast carcinoma cell death. Our results show that increasing ZNF32 expression in MCF-7 cells inhibits autophagy initiation by activating the AKT/mTOR pathway, and further reduced autophagy-associated cell death and maintained MCF-7 cell survival. Conversely, impairing ZNF32 expression by transfecting ZNF32 siRNA strongly promoted autophagy, further augmenting autophagy-associated cell death. Furthermore, correlations between ZNF32 and autophagy were observed in both MCF-7 xenograft tumors and in breast cancer...Continue Reading

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Citations

Oct 12, 2017·Frontiers in Cellular and Infection Microbiology·Haichang YinHongyan Chen
Apr 11, 2020·Redox Biology·Juan Diego Hernández-CamachoIgnacio Navas-Enamorado
Jun 24, 2021·Journal of Molecular Neuroscience : MN·Shan WenXifan Mei
Dec 30, 2017·ACS Applied Materials & Interfaces·Xuerui ChenWenjun Fang

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Methods Mentioned

BETA
xenograft
electrophoresis
ELISA
Flow Cytometry
Transfection
dissection

Software Mentioned

Quantity One
FlowJo
SPSS

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