ZnJ2 Is a Member of a Large Chaperone Family in the Chloroplast of Photosynthetic Organisms that Features a DnaJ-Like Zn-Finger Domain

Frontiers in Molecular Biosciences
Lior DoronMichal Shapira

Abstract

Photosynthesis is performed by large complexes, composed of subunits encoded by the nuclear and chloroplast genomes. Assembly is assisted by general and target-specific chaperones, but their mode of action is yet unclear. We formerly showed that ZnJ2 is an algal chaperone resembling BSD2 from land plants. In algae, it co-migrates with therbcLtranscript on chloroplast polysomes, suggesting it contributes to thede-novosynthesis of RbcL (Doron et al., 2014). ZnJ2 contains four CXXCXGXG motifs, comprising a canonical domain typical also of DnaJ-type I (DNAJA). It contributes to the binding of protein substrates to DnaK and promotes an independent oxidoreductase activity (Mattoo et al., 2014). To examine whether ZnJ2 has oxidoreductase activity, we used the RNaseA assay, which measures the oxidation-dependent reactivation of reduced-denatured RNaseA. Although ZnJ2 assisted the native refolding of reduced-denatured RNaseA, its activity was restricted to an oxidizing environment. Thus, ZnJ2 did not carry the exclusive responsibility for the formation of disulfide bridges, but contributed to the stabilization of its target polypeptides, until they reached their native state. A ZnJ2 cysteine deficient mutant maintained a similar holding...Continue Reading

References

Jan 1, 1990·Annual Review of Cell Biology·A A Gatenby, R J Ellis
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V P Pigiet, B J Schuster
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
May 26, 1995·Journal of Molecular Biology·J S McCartyB Bukau
Jun 21, 1996·The Journal of Biological Chemistry·B BaneckiM Zylicz
Feb 1, 1997·Current Opinion in Structural Biology·J Martin, F U Hartl
Apr 16, 1998·Methods in Enzymology·J HorwitzM P Bova
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Jul 1, 1995·The Plant Cell·S. Gutteridge, A. A. Gatenby
Sep 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·C B ANFINSENF H WHITE
Dec 20, 2003·Nature·Christopher M Dobson
Aug 16, 2005·Progress in Biophysics and Molecular Biology·Alexandra P CarvalhoMaria J Ramos
Apr 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·Katia Wostrikoff, David Stern
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Dec 21, 2007·Biochimica Et Biophysica Acta·Christian Appenzeller-Herzog, Lars Ellgaard
Mar 12, 2008·Biochimica Et Biophysica Acta·Carsten BerndtArne Holmgren
Jul 30, 2008·Cell Stress & Chaperones·Harm H KampingaLawrence E Hightower
Jan 16, 2010·Nature·Cuimin LiuManajit Hayer-Hartl
May 5, 2010·Nucleic Acids Research·Mickael GoujonRodrigo Lopez
Jul 24, 2010·Nature Reviews. Molecular Cell Biology·Harm H Kampinga, Elizabeth A Craig
Oct 19, 2010·Nature Chemical Biology·Sandeep K SharmaPierre Goloubinoff
May 6, 2011·The Journal of Biological Chemistry·Despoina A I MavridouChristina Redfield
Oct 4, 2014·The Plant Journal : for Cell and Molecular Biology·Leila FeizDavid B Stern

❮ Previous
Next ❯

Citations

Jan 28, 2021·International Journal of Molecular Sciences·Richa Amiya, Michal Shapira

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleotide exchange
protein folding

Software Mentioned

ClustalW2
Clustal Omega
GeneDoc
Gonnet
Clustal W2
Clustal W2 _ Phylogeny
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.