ZnO Nanoparticles Treatment Induces Apoptosis by Increasing Intracellular ROS Levels in LTEP-a-2 Cells

BioMed Research International
Caixia WangYinglu Ji

Abstract

Owing to the wide use of novel nanoparticles (NPs) such as zinc oxide (ZnO) in all aspects of life, toxicological research on ZnO NPs is receiving increasing attention in these days. In this study, the toxicity of ZnO NPs in a human pulmonary adenocarcinoma cell line LTEP-a-2 was tested in vitro. Log-phase cells were exposed to different levels of ZnO NPs for hours, followed by colorimetric cell viability assay using tetrazolium salt and cell survival rate assay using trypan blue dye. Cell morphological changes were observed by Giemsa staining and light microscopy. Apoptosis was detected by using fluorescence microscopy and caspase-3 activity assay. Both intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) were examined by a microplate-reader method. Results showed that ZnO NPs (≥ 0.01 μg/mL) significantly inhibited proliferation (P < 0.05) and induced substantial apoptosis in LTEP-a-2 cells after 4 h of exposure. The intracellular ROS level rose up to 30-40% corresponding to significant depletion (approximately 70-80%) in GSH content in LTEP-a-2 cells (P < 0.05), suggesting that ZnO NPs induced apoptosis mainly through increased ROS production. This study elucidates the toxicological mechanism of ZnO NPs i...Continue Reading

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Citations

Feb 20, 2020·Materials·Ana Rita PinhoMaria de Lourdes Pereira
May 18, 2020·Journal of Toxicology and Environmental Health. Part a·Jéssica Schveitzer KöerichWilliam Gerson Matias
Oct 9, 2016·Clinical and Experimental Pharmacology & Physiology·Hesham Fathy Hassan HassanBasim Anwar Shehata Messiha
Jun 12, 2020·Biomimetics·Maria P Nikolova, Murthy S Chavali
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Mar 16, 2021·ACS Applied Materials & Interfaces·Irene VillaAngelo Monguzzi

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Methods Mentioned

BETA
dynamic light scattering

Software Mentioned

Origin
SPSS16

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis