ZnT-1 expression in astroglial cells protects against zinc toxicity and slows the accumulation of intracellular zinc

Glia
Christiane NolteArie Moran

Abstract

Zinc ions are emerging as an important factor in the etiology of neurodegenerative disorders and in brain damage resulting from ischemia or seizure activity. High intracellular levels of zinc are toxic not only to neurons but also to astrocytes, the major population of glial cells in the brain. In the present study, the role of ZnT-1 in reducing zinc-dependent cell damage in astrocytes was assessed. Zinc-dependent cell damage was apparent within 2 h of exposure to zinc, and occurred within a narrow range of approximately 200 microM. Pretreatment with sublethal concentrations of zinc rendered astrocytes less sensitive to toxic zinc levels, indicating that preconditioning protects astrocytes from zinc toxicity. Fluorescence cell imaging revealed a steep reduction in intracellular zinc accumulation for the zinc-pretreated cells mediated by L-type calcium channels. Heterologous expression of ZnT-1 had similar effects; intracellular zinc accumulation was slowed down and the sensitivity of astrocytes to toxic zinc levels was reduced, indicating that this is specifically mediated by ZnT-1 expression. Immunohistochemical analysis demonstrated endogenous ZnT-1 expression in cultured astroglia, microglia, and oligodendrocytes. Pretreatme...Continue Reading

References

Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J Streit
Jan 1, 1989·International Review of Neurobiology·C J Frederickson
Jan 1, 1988·Neuroscience·D W ChoiJ Koh
Jun 1, 1972·Journal of Neurochemistry·I L Crawford, J D Connor
Jun 1, 1980·The Journal of Cell Biology·K D McCarthy, J de Vellis
Apr 19, 1984·Nature·S Y Assaf, S H Chung
Jan 1, 1993·Neuron·J H WeissD W Choi
Jan 1, 1996·Annual Review of Physiology·L S King, P Agre
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·E P Huang
Apr 8, 1998·Annual Review of Neuroscience·D W Choi, J Y Koh
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·R J McMahon, R J Cousins
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·T B ColeR D Palmiter
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·L L PearceE S Levitan
Jun 14, 2000·Neurology·J MoncayoG van Melle
Aug 23, 2000·The Journal of Biological Chemistry·S J LangmadeG K Andrews
Aug 31, 2000·Neurobiology of Disease·K E DineleyI J Reynolds
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·C J Frederickson, A I Bush
May 2, 2002·The Journal of Comparative Neurology·Israel SeklerWilliam F Silverman
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Stefano L SensiJohn H Weiss
Oct 3, 2003·Trends in Neurosciences·Bruce RansomMaiken Nedergaard

❮ Previous
Next ❯

Citations

Jun 3, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ehud OhanaMichal Hershfinkel
Jun 16, 2010·Journal of Neural Transmission·Glenda M BishopStephen R Robinson
Sep 22, 2009·The Journal of Biological Chemistry·Shiri LevyArie Moran
Sep 21, 2010·Antioxidants & Redox Signaling·Mandar A Aras, Elias Aizenman
Jul 12, 2007·Molecular Medicine·Israel SeklerWilliam F Silverman
Jun 22, 2014·Metallomics : Integrated Biometal Science·Eden ShustermanArie Moran
Aug 29, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Zhelong Xu, Juan Zhou
Feb 21, 2013·Metallomics : Integrated Biometal Science·Atsushi TakedaHaruna Tamano
Feb 12, 2013·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Mansour SargaziNorman B Roberts
May 24, 2008·Autonomic Neuroscience : Basic & Clinical·Hui-Ling GaoZhan-You Wang
Aug 28, 2007·Brain Research Bulletin·Li ZhangZhan-You Wang
Mar 27, 2007·Free Radical Biology & Medicine·Glenda M BishopStephen R Robinson
Mar 21, 2016·Cell Calcium·Kirill Kiselyov, Shmuel Muallem
Mar 17, 2009·Journal of Cellular Physiology·Tehila Kaisman-ElbazWilliam F Silverman
Jul 21, 2006·Glia·Emilio VareaXavier Ponsoda
Nov 1, 2007·Journal of Cardiovascular Electrophysiology·Yoram EtzionAmos Katz
Feb 14, 2012·Glia·Israel Sekler, William F Silverman
Apr 5, 2013·Journal of Neuroscience Research·Joshua T JohnstoneJohn R Bethea
May 1, 2010·Journal of Cellular Physiology·Natalya KarolWilliam F Silverman
Sep 29, 2004·Biochemical and Biophysical Research Communications·Dror SegalIsrael Sekler
Apr 22, 2006·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Frederik MuylleRonny Blust
Jan 21, 2015·Journal of Neurochemistry·Gabriela K Popescu
Mar 31, 2015·Expert Opinion on Therapeutic Targets·Atsushi Takeda, Haruna Tamano
May 6, 2006·Biochimica Et Biophysica Acta·David J Eide
Jul 11, 2014·Frontiers in Aging Neuroscience·Sara M HancockPaul A Adlard
Jul 10, 2016·Archives of Biochemistry and Biophysics·Atsushi Takeda, Hanuna Tamano
Jan 17, 2017·Metallomics : Integrated Biometal Science·Eden ShustermanArie Moran
Jun 1, 2007·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Andrew P Smith, Nancy M Lee
Oct 15, 2009·Nature Reviews. Neuroscience·Stefano L SensiIsrael Sekler
Jun 24, 2006·The Journal of Biological Chemistry·Robert J CousinsLouis A Lichten
Apr 25, 2008·Critical Reviews in Toxicology·Edward J Calabrese
May 11, 2012·American Journal of Physiology. Cell Physiology·Merav MorYoram Etzion
Mar 20, 2015·The Journal of Biological Chemistry·Jian XieChunyu Wang
Sep 1, 2019·The Journal of Biological Chemistry·Yukina Nishito, Taiho Kambe

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