ZO-1 reorganization and myofibroblast transformation of corneal endothelial cells after freeze injury in the cat

Experimental Eye Research
W M PetrollJ V Jester

Abstract

Corneal endothelial wound healing following scrape injury in the rabbit and cat is characterized by cell spreading and maintenance of a normal endothelial phenotype consisting of apically-localized, circumferential microfilament bands and cell border-associated ZO-1, a tight junction protein and marker for endothelial differentiation. In contrast, after freeze injury in the rat and rabbit endothelial cells develop basally organized microfilament bundles (stress fibers), and appear to proliferate and form a multilayered zone at the wound margin. The purpose of the present study was to determine if similar phenotypic changes are observed after freeze injury in the cat corneal endothelium, which like human, normally has limited growth potential. In addition, changes in ZO-1 and alpha-smooth muscle actin (a marker for myofibroblast transformation) distribution were evaluated for the first time following freeze injury. In vivo endothelial healing of standard 3 mm diameter freeze injury was evaluated at 4 hr, 12 hr, 24 hr, 48 hr, 3 days and 5 days after injury in 22 cat eyes. Corneas were stained with phalloidin, propidium iodide, and anti-ZO-1, anti-alpha-smooth muscle-specific actin or anti-fibronectin antibodies. Protein organizat...Continue Reading

Citations

Nov 26, 2009·Veterinary Ophthalmology·Kathern E MyrnaChristopher J Murphy
Jul 22, 2015·Progress in Retinal and Eye Research·Alexander V Ljubimov, Mehrnoosh Saghizadeh
Jan 21, 2015·Eye & Contact Lens·Jin Woo NohYoung Joo Shin
Aug 21, 2016·The British Journal of Ophthalmology·Maninder BhogalBruce D Allan
Aug 10, 2007·American Journal of Physiology. Cell Physiology·Silvina GrassoSilvia Chifflet
Jun 9, 2017·Journal of Ophthalmology·Alina AkhbanbetovaAndrew J Quantock
Jun 25, 2020·International Journal of Molecular Sciences·Jin Sun HwangYoung Joo Shin
Oct 13, 2017·Experimental Eye Research·Hidetaka MiyagiChristopher J Murphy

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