Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways

Journal of Experimental & Clinical Cancer Research : CR
Li WangFeng Luo

Abstract

Zoledronic acid is the most potent osteoclast inhibitor and is widely used for advanced cancer patients with bone metastasis, but its role on cancer stem cells (CSCs) remains unclear. In the present study, we aimed to identify the stemness phenotypic characteristics of CSCs derived from cervical cancer cells and explore the anti-cancer efficiency of zoledronic acid on these cells, as well as the possible molecular mechanisms. Stemness phenotypic identification of cervical cancer cells derived CSCs was performed via sphere formation efficiency (SFE), tumorigenesis, immunofluorescence staining, Transwell assay, and western blot. Anti-cancer efficiency of zoledronic acid on these cells (including proliferation, stemness phenotype, apoptosis, and cell cycle) was carried out through MTT assay, SFE, transwell, DAPI staining, flow cytometry, immunofluorescence, TUNEL staining, and western blot, both in vitro and in vivo. Enhanced self-renewal ability, including SFE and tumorigenesis, was verified in cervical cancer cells derived CSCs compared to parental cervical cancer cells. Specifically, the expression of ALDH1, Sox2, CD49f, Nanog, and Oct4 was significantly up-regulated in cervical cancer cells derived CSCs. Furthermore, enhanced ...Continue Reading

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Citations

Mar 20, 2020·The Journal of Pharmacy and Pharmacology·Hai LinHongyu Liu
Feb 28, 2020·Experimental and Therapeutic Medicine·Changde LiuYuhua Ying
Jun 13, 2020·Frontiers in Cellular Neuroscience·Jianhua ZhouJinghui Huang
May 28, 2020·Cancers·Ángela ÁyenHouria Boulaiz
Dec 15, 2020·World Journal of Stem Cells·Xu Gao, Qiong-Zhu Dong
Apr 8, 2021·Journal of Gynecologic Oncology·Mengdi HeHaiou Liu
Apr 6, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hyesu JeonKyung-Sun Heo
Jul 9, 2021·Frontiers in Cell and Developmental Biology·Martina Mang Leng Lei, Terence Kin Wah Lee
Nov 7, 2020·Journal of Experimental & Clinical Cancer Research : CR·Qiang TangMaojun Di

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Methods Mentioned

BETA
flow cytometry
xenografts

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