PMID: 2567158May 1, 1989Paper

Zonation of hepatic lipogenic enzymes identified by dual-digitonin-pulse perfusion

The Biochemical Journal
J L EvansL A Witters

Abstract

The zonal distribution within rat liver of acetyl-CoA carboxylase, ATP citrate-lyase and fatty acid synthase, the principal enzymes of fatty acid synthesis, was investigated by using dual-digitonin-pulse perfusion. Analysis of enzyme mass by immunoblotting revealed that, in normally feeding male rats, the periportal/perivenous ratio of acetyl-CoA carboxylase mass was 1.9. The periportal/perivenous ratio of ATP citrate-lyase mass was 1.4, and fatty acid synthase exhibited the largest periportal/perivenous mass gradient, having a ratio of 3.1. This pattern of enzyme distribution was observed in male rats only; in females, the periportal/perivenous ratio of enzyme mass was nearly equal. The periportal/perivenous gradients for acetyl-CoA carboxylase, ATP citrate-lyase and fatty acid synthase observed in fed (and fasted) males were abolished when animals were fasted (48 h) and refed (30 h) with a high-carbohydrate/low-fat diet. As determined by enzyme assay of eluates obtained from the livers of normally feeding male rats, there is also periportal zonation of acetyl-CoA carboxylase activity, expressed either as units per mg of eluted protein or units per mg of acetyl-CoA carboxylase protein, suggesting the existence of gradients in ...Continue Reading

Citations

Apr 30, 2016·Nutrition & Metabolism·Ping ZhangFeiruo Huang
Aug 27, 2002·Proceedings of the National Academy of Sciences of the United States of America·Y LeeR H Unger
Sep 3, 1990·FEBS Letters·A BianchiL A Witters
Oct 24, 2006·The FEBS Journal·Albert BraeuningMichael Schwarz
Feb 15, 1992·European Journal of Biochemistry·C T KnudsenB Quistorff
Dec 22, 1999·The American Journal of Physiology·M A PuchowiczH Brunengraber
Oct 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Eun-Kyoung KimGabriele V Ronnett
Apr 1, 1991·The American Journal of Physiology·W M Bennet, M W Haymond
Apr 15, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nikolaus BerndtMadlen Matz-Soja
May 15, 2002·Brain Research. Molecular Brain Research·Jérôme SallesBertrand Garbay

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