Zonisamide upregulates neuregulin-1 expression and enhances acetylcholine receptor clustering at the in vitro neuromuscular junction.

Neuropharmacology
Taro InoueKinji Ohno

Abstract

Decreased acetylcholine receptor (AChR) clustering compromises signal transmission at the neuromuscular junction (NMJ) in myasthenia gravis, congenital myasthenic syndromes, and motor neuron diseases. Although the enhancement of AChR clustering at the NMJ is a promising therapeutic strategy for these maladies, no drug is currently available for this enhancement. We previously reported that zonisamide (ZNS), an anti-epileptic and anti-Parkinson's disease drug, enhances neurite elongation of the primary spinal motor neurons (SMNs). As nerve sprouting occurs to compensate for the loss of AChR clusters in human diseases, we examined the effects of ZNS on AChR clustering at the NMJ. To this end, we established a simple and quick co-culture system to reproducibly make in vitro NMJs using C2C12 myotubes and NSC34 motor neurons. ZNS at 1-20 μM enhanced the formation of AChR clusters dose-dependently in co-cultured C2C12 myotubes but not in agrin-treated single cultured C2C12 myotubes. We observed that molecules that conferred responsiveness to ZNS were not secreted into the co-culture medium. We found that 10 μM ZNS upregulated the expression of neuregulin-1 (Nrg1) in co-cultured cells but not in single cultured C2C12 myotubes or singl...Continue Reading

References

Jul 1, 1992·Developmental Dynamics : an Official Publication of the American Association of Anatomists·N R CashmanJ P Antel
May 13, 1994·Brain Research. Developmental Brain Research·P H BonnerR S Baker
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G JonesH R Brenner
Feb 20, 1999·Archives of Neurology·A G EngelS M Sine
May 21, 1999·The Journal of Biological Chemistry·D KimM S Kang
Dec 5, 2003·Journal of Molecular and Cellular Cardiology·Ryuji FukazawaDouglas B Sawyer
Jan 23, 2004·Experimental Neurology·Lindsey R FischerJonathan D Glass
Aug 25, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Thomas W GouldRonald W Oppenheim
Oct 4, 2006·Neuron·Séverine BoilléeDon W Cleveland
Mar 6, 2007·Journal of Neuroscience Research·Mendell Rimer
Oct 14, 2008·Cell·Natalie KimSteven J Burden
Oct 30, 2008·Neuron·Bin ZhangLin Mei
Feb 16, 2011·Brain Research·Mohammed Emamussalehin ChoudhuryMasahiro Nomoto
Mar 8, 2011·Cell·Steven J Burden
Apr 27, 2011·Journal of Neurochemistry·Thomas CarlssonGünter U Höglinger
Nov 4, 2011·Stem Cell Reviews and Reports·Marianne StockmannTobias M Boeckers
Dec 21, 2011·The Journal of Cell Biology·Nadine SchmidtHans Rudolf Brenner
Jun 25, 2013·The EMBO Journal·Cyril CheretCarmen Birchmeier
Sep 18, 2013·Biomaterials·Yuya MorimotoShoji Takeuchi
May 24, 2016·Neural Regeneration Research·Kinji OhnoBisei Ohkawara
Jan 1, 2000·Pflügers Archiv : European journal of physiology·Tomaž MaršZoran Grubič
Aug 22, 2017·Expert Opinion on Therapeutic Targets·Kinji OhnoMikako Ito
Aug 6, 2020·Scientific Reports·Shunsuke KanbaraKinji Ohno
Mar 7, 2021·International Journal of Molecular Sciences·Bisei OhkawaraKinji Ohno

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