Zonula occludens-1 (ZO-1) is involved in morula to blastocyst transformation in the mouse.

Developmental Biology
Hehai WangB C Paria

Abstract

It is unknown whether or not tight junction formation plays any role in morula to blastocyst transformation that is associated with development of polarized trophoblast cells and fluid accumulation. Tight junctions are a hallmark of polarized epithelial cells and zonula occludens-1 (ZO-1) is a known key regulator of tight junction formation. Here we show that ZO-1 protein is first expressed during compaction of 8-cell embryos. This stage-specific appearance of ZO-1 suggests its participation in morula to blastocyst transition. Consistent with this idea, we demonstrate that ZO-1 siRNA delivery inside the blastomeres of zona-weakened embryos using electroporation not only knocks down ZO-1 gene and protein expressions, but also inhibits morula to blastocyst transformation in a concentration-dependent manner. In addition, ZO-1 inactivation reduced the expression of Cdx2 and Oct-4, but not ZO-2 and F-actin. These results provide the first evidence that ZO-1 is involved in blastocyst formation from the morula by regulating accumulation of fluid and differentiation of nonpolar blastomeres to polar trophoblast cells.

References

Mar 1, 1991·Journal of Reproduction and Fertility·J A Lawitts, J D Biggers
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B C Paria, S K Dey
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B C PariaS K Dey
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·L LarueR Kemler
Feb 3, 2000·Nature Cell Biology·F Wianny, M Zernicka-Goetz
Dec 5, 2000·Molecular Biology of the Cell·M SaitouS Tsukita
Mar 10, 2001·Genes & Development·P A Sharp
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
Aug 7, 2001·Frontiers in Bioscience : a Journal and Virtual Library·T P FlemingI Fesenko
Apr 12, 2002·Genesis : the Journal of Genetics and Development·Joanna B GrabarekMagdalena Zernicka-Goetz
Jul 12, 2002·Nature·Gregory J Hannon
May 14, 2003·The Journal of Cell Biology·Takehiro NittaShoichiro Tsukita
May 1, 1963·The Journal of Cell Biology·M G FARQUHAR, G E PALADE
Mar 25, 2004·Molecular Reproduction and Development·Seiki HaraguchiAkira Seto
Jul 24, 2004·Seminars in Cell & Developmental Biology·Martin H Johnson, Josie M L McConnell
Aug 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexander GowBechara Kachar
Dec 31, 2005·BMC Developmental Biology·Miguel L SoaresMagdalena Zernicka-Goetz
Jun 15, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Yojiro YamanakaJanet Rossant
Aug 22, 2006·Nature Materials·Mireille M A E ClaessensAndreas R Bausch
Oct 31, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·John T BenjaminLawrence S Prince

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Citations

Mar 16, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Chang-Gi HurDawon Kang
Sep 6, 2014·Journal of Human Reproductive Sciences·Rocío Rivera EgeaAlex C Varghese
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mark LommelSabine Strahl
Feb 25, 2010·Development·Lei LiJurrien Dean
May 28, 2013·In Vitro Cellular & Developmental Biology. Animal·Bo-hao ChangYong Zhang
Feb 23, 2011·Zygote : the Biology of Gametes and Early Embryos·Shangdan XuMasashi Miyake
Dec 22, 2015·The American Journal of Pathology·Deepak VenkateshTanya N Mayadas
Sep 9, 2015·Seminars in Cell & Developmental Biology·John S BigginsShankar Srinivas
May 30, 2009·Molecular Reproduction and Development·Yusuke Marikawa, Vernadeth B Alarcón
Sep 21, 2011·Journal of Neurochemistry·Alexander JacobJessy J Alexander
May 9, 2012·Current Opinion in Genetics & Development·Hervé AcloqueM Angela Nieto
Aug 22, 2016·Journal of Assisted Reproduction and Genetics·Wenhong MaXiaoyan Liang
Jun 21, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Hui PengWenchang Zhang
Jul 24, 2020·Development·Berenika Płusa, Anna Piliszek
Sep 7, 2020·Journal of Assisted Reproduction and Genetics·Angelo Tocci
Apr 16, 2015·Reproductive Medicine and Biology·Agnieszka Jedrusik
Jul 7, 2017·Biology of the Cell·Jean-Léon Maître
Feb 2, 2021·Methods : a Companion to Methods in Enzymology·Marina Gertsenstein, Lauryl M J Nutter
Jul 28, 2020·Current Opinion in Cell Biology·Özge Özgüç, Jean-Léon Maître
May 1, 2021·Nature Reviews. Molecular Cell Biology·Hui Yi Grace Lim, Nicolas Plachta
May 1, 2021·Journal of Developmental Biology·Bilal Ahmad HakimMonika Sachdev
Aug 26, 2021·STAR Protocols·Ronghui Li, Juan Carlos Izpisua Belmonte
Sep 30, 2021·Cell and Tissue Research·Ecem YildirimAylin Yaba
Oct 19, 2019·Cell·Ronghui LiJuan Carlos Izpisua Belmonte

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