ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans

Frontiers in Physiology
Lorenzo BertoloneAngelo D'Alessandro

Abstract

As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored human (n = 21, 10 males, 11 females), olive baboon (n = 20), and rhesus macaque (n = 20) red blood cells at baseline and upon 42 days of storage under blood bank conditions. The results indicated similarities and differences across species, which ultimately resulted in a differential propensity to undergo morphological alterations and lyse as a function of the duration of refrigerated storage. Focusing on purine oxidation, carboxylic acid, fatty acid, and arginine metabolism further highlighted species-specific metabolic wiring. For example, through a combination of steady state measurements and 13C615N4-arginine tracing experiments, we report an increase in arginine catabolism into ornithine in humans, suggestive of species-specific arginase 1 activity and nitric oxide synthesis-an observation that may impact the translatability of cardiovascular disease studies carried out in non-human primates (NHPs). Finally, we correlated metabol...Continue Reading

References

May 5, 2005·Blood·Michael C MahaneyOrah S Platt
Dec 22, 2005·Blood·Petra KleinbongardMalte Kelm
Apr 14, 2007·Science·UNKNOWN Rhesus Macaque Genome Sequencing and Analysis ConsortiumAnn S Zwieg
Oct 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Elliott Bennett-GuerreroTimothy J McMahon
Oct 6, 2009·Genome Research·Adam Siepel
Aug 29, 2012·Blood Transfusion = Trasfusione Del Sangue·Angelo D'AlessandroLello Zolla
Jan 1, 2011·Comprehensive Physiology·Allan Doctor, Jonathan S Stamler
Nov 1, 2013·ILAR Journal·Laura A CoxJohn L VandeBerg
Sep 23, 2014·Transfusion·Jose A CancelasMajid Zia
Dec 15, 2015·Transfusion·Travis NemkovAngelo D'Alessandro
Sep 21, 2016·Journal of Proteome Research·Angelo D'AlessandroRobert C Roach
Nov 5, 2016·Transfusion·Angelo D'AlessandroKirk C Hansen
Mar 7, 2017·Blood Transfusion = Trasfusione Del Sangue·Harvey G Klein
Mar 7, 2017·Blood Transfusion = Trasfusione Del Sangue·Angelo D'alessandroKirk C Hansen
May 4, 2017·The Journal of Trauma and Acute Care Surgery·Nathan ClendenenAngelo DʼAlessandro
Jun 18, 2017·Cell Death and Differentiation·Lars Kaestner, Giampaolo Minetti
Dec 15, 2017·Clinical Pharmacology and Therapeutics·James D ReynoldsJonathan S Stamler
May 16, 2018·Nucleic Acids Research·Jasmine ChongJianguo Xia
Jun 7, 2018·Rapid Communications in Mass Spectrometry : RCM·Biswapriya B MisraMichael Olivier
Jan 18, 2019·Blood Transfusion = Trasfusione Del Sangue·Tatsuro YoshidaAngelo D'alessandro
Mar 21, 2019·Blood Advances·Angelo D'AlessandroMark T Gladwin
May 24, 2019·Methods in Molecular Biology·Gretchen L SeimJing Fan
May 24, 2019·Methods in Molecular Biology·Shubhra AgrawalSwetabh Pathak

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Vassilis L TzounakasMarianna H Antonelou

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Methods Mentioned

BETA
PCA
deamination
blood drawn

Software Mentioned

ZOOMICS
PLS
El
GENE
MAVEN
MetaboAnalyst
DA
GraphPad Prism

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