Zygotic genome activation triggers the DNA replication checkpoint at the midblastula transition

Cell
Shelby A Blythe, Eric F Wieschaus

Abstract

A conserved feature of the midblastula transition (MBT) is a requirement for a functional DNA replication checkpoint to coordinate cell-cycle remodeling and zygotic genome activation (ZGA). We have investigated what triggers this checkpoint during Drosophila embryogenesis. We find that the magnitude of the checkpoint scales with the quantity of transcriptionally engaged DNA. Measuring RNA polymerase II (Pol II) binding at 20 min intervals over the course of ZGA reveals that the checkpoint coincides with widespread de novo recruitment of Pol II that precedes and does not require a functional checkpoint. This recruitment drives slowing or stalling of DNA replication at transcriptionally engaged loci. Reducing Pol II recruitment in zelda mutants both reduces replication stalling and bypasses the requirement for a functional checkpoint. This suggests a model where the checkpoint functions as a feedback mechanism to remodel the cell cycle in response to nascent ZGA.

Citations

Jan 18, 2018·PLoS Genetics·Yi Xie, J Todd Blankenship
Oct 27, 2018·PLoS Genetics·Tanguy LucasNathalie Dostatni
Nov 6, 2018·Development·Jumana Alhaj AbedRichard S Jones
Jun 14, 2019·Development·Nadine L VastenhouwHoward D Lipshitz
Sep 6, 2019·G3 : Genes - Genomes - Genetics·Boyang LiuJörg Großhans
Feb 6, 2020·Scientific Reports·Christopher Amourda, Timothy E Saunders
Nov 4, 2017·The Journal of Experimental Biology·Brent L LockwoodKristi L Montooth
Nov 22, 2017·Development·Michael MeierJulia A Horsfield
Apr 5, 2018·Journal of Developmental Biology·Fabio Alexis Lefebvre, Éric Lécuyer
Sep 13, 2019·Development·Sudarshan ChariAmanda A Amodeo
Feb 16, 2020·Nature Communications·Rodrigo G Arzate-MejíaFélix Recillas-Targa
Jan 21, 2017·Genes·Chames KermiDomenico Maiorano
Jan 25, 2017·Proceedings of the National Academy of Sciences of the United States of America·Hanieh Falahati, Eric Wieschaus
Jul 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Ann Boija, Mattias Mannervik
Jul 31, 2019·Nature Structural & Molecular Biology·Matthew WootenXin Chen
May 12, 2020·Journal of Experimental Botany·Aline V ProbstCrisanto Gutierrez
Feb 18, 2016·ELife·Zehra Ali-Murthy, Thomas B Kornberg
May 14, 2020·Molecular Biology of the Cell·Kaitlin P KoreskiRobert J Duronio
Aug 26, 2017·Genes & Development·Leila E RiederErica N Larschan
Sep 24, 2019·Cell Cycle·Boyang Liu, Jörg Großhans
Feb 3, 2021·Cellular and Molecular Life Sciences : CMLS·Darya ChetverinaPaul Schedl
Dec 26, 2019·Cell Reports·Leila Elizabeth RiederErica Nicole Larschan
Mar 7, 2020·Developmental Cell·Shannon E KeenanStanislav Y Shvartsman
Apr 2, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sahla SyedAmanda A Amodeo
Apr 3, 2021·Nature Genetics·Elizabeth Ing-SimmonsJuan M Vaquerizas
Apr 10, 2021·The Journal of Chemical Physics·Yonghyun Song, Changbong Hyeon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.