Zymogram and Preliminary Characterization of Lactobacillus helveticus Autolysins.

Applied and Environmental Microbiology
Florence Valence, Sylvie Lortal

Abstract

The autolysins of Lactobacillus helveticus ISLC5 were detected and partially characterized by renaturing sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). By using lyophilized Micrococcus luteus cells or heated whole cells of L. helveticus ISLC5 (0.2% [wt/vol]) as a substrate, several lytic activities were detected in the whole-cell SDS extract of strain ISLC5 (i) one activity at 42.4 kDa, which was named autolysin A, and (ii) six other activities having very similar molecular weights (29.1, 29.6, 30, 30.8, 31.7, and 32.8 kDa), which were named autolysins B (B1 through B6, respectively). As regards the temporal distribution of the enzymes, autolysins A and B were detected in the cells harvested from the beginning of the exponential growth phase. Autolysin A appeared to be associated only with viable cells, whereas the autolysins B remained associated with the cell envelope several days after the complete loss of culture viability. When SDS-treated walls of L. helveticus ISLC5 were used as a substrate, a supplementary lytic activity appeared at 37.5 kDa; it was considered a peptidoglycan hydrolase, since it was not able to induce lysis of whole-cell substrate. The autolysi...Continue Reading

References

Oct 1, 1979·Journal of General Microbiology·R Williamson, J B Ward
Feb 1, 1978·Journal of Bacteriology·R P HinksG D Shockman
Jan 1, 1992·Advances in Microbial Physiology·P Messner, U B Sleytr
Aug 1, 1989·Canadian Journal of Microbiology·D Leclerc, A Asselin
Oct 1, 1988·Molecular & General Genetics : MGG·C PotvinG Bellemare
Dec 1, 1973·Journal of Bacteriology·M L HigginsG D Shockman
Dec 1, 1972·Bacteriological Reviews·K H Schleifer, O Kandler
Feb 1, 1973·Applied Microbiology·W C Brown
Jan 22, 1971·Nature·C Forsberg, H J Rogers
Mar 27, 1970·Biochemical and Biophysical Research Communications·H M PooleyG D Shockman
Mar 1, 1968·Applied Microbiology·A OterholmL D Witter
Feb 1, 1961·Journal of General Microbiology·J BADDILEY, A L DAVISON

❮ Previous
Next ❯

Citations

Oct 1, 1996·Antonie van Leeuwenhoek·M J Gasson
Feb 2, 2000·Applied and Environmental Microbiology·C Husson-KaoM P Chapot-Chartier
Jun 1, 2000·FEMS Microbiology Letters·C MercierS Kulakauskas
Aug 5, 2000·Applied and Environmental Microbiology·M C Martínez-CuestaG Buist
Jul 17, 2016·Applied and Environmental Microbiology·Brant R Johnson, Todd R Klaenhammer
Oct 22, 2005·Journal of Applied Microbiology·N RaddadiD Daffonchio
Sep 8, 2006·Applied and Environmental Microbiology·Maurice NdagijimanaM Elisabetta Guerzoni
Oct 22, 2008·Applied and Environmental Microbiology·Mariano Prado AcostaSandra M Ruzal
Jan 13, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·M ArtiniL Selan
Apr 18, 2014·Journal of Industrial Microbiology & Biotechnology·Feng HanMuhammad Naseem Khan
Apr 1, 2000·Applied and Environmental Microbiology·G GiraffaE Neviani
Mar 17, 2009·Applied and Environmental Microbiology·M GenayS Lortal
Feb 6, 2018·Probiotics and Antimicrobial Proteins·Izildinha MorenoRenata Bromberg
Dec 15, 2020·Applied and Environmental Microbiology·Kirstin I ArendChristine Kaimer
Mar 15, 2006·Applied and Environmental Microbiology·H R RiepeL L McKay

❮ Previous
Next ❯

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.