Apr 25, 2007

Zymographic analysis and characterization of MMP-2 and -9 forms in human sound dentin

Journal of Dental Research
Annalisa MazzoniL Breschi

Abstract

The role and function of dentin matrix metalloproteinases (MMPs) are not well-understood, but they may play a key role in dentinal caries and the degradation of resin-bonded dentin matrices. To test the null hypothesis that MMP-9 is not found in dentin matrix, we used gelatin zymography to extract and isolate all molecular forms of gelatinolytic MMPs in demineralized mature sound dentin powder obtained from extracted human molars, characterizing and identifying the enzymes by Western blotting. Gelatinolytic MMPs were detected in extracts of demineralized dentin matrix and identified as MMP-2 and MMP-9. Acidic extracts (pH 2.3) yielded 3-8 times more MMP activity than did EDTA (pH 7.4). Their activation may contribute to dentin matrix degradation, which occurs during caries progression and following resin bonding. Inhibition of MMP-2 and -9 proteolytic activity may slow caries progression and increase the durability of resin-dentin bonds.

  • References28
  • Citations117

References

Mentioned in this Paper

Enzymes, antithrombotic
Western Blotting
SDS-PAGE
Extracellular Matrix
Matrix Metalloproteinase 2
MMP-2 Activity
Proteolytic Enzyme
MMP-9 Activity
Matrix Metalloproteinase 9
Western Blot

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