Zymography of metalloproteinases

Current Protocols in Protein Science
Linda Troeberg, Hideaki Nagase

Abstract

Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).

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Citations

Aug 23, 2012·Molecular Cancer Research : MCR·Brett C DiNataleGary H Perdew
Jul 3, 2013·International Journal of Oncology·Mitsunori TodaToshihiro Akisue
Aug 24, 2010·Neurochemical Research·Hyo Sang JangJeffrey A Greenwood
Jan 27, 2019·Scientific Reports·Konstantin KarmilinWalter Stöcker
Apr 22, 2020·Osteoarthritis and Cartilage·A-C SevermannA Vortkamp

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