Zyxin controls migration in epithelial-mesenchymal transition by mediating actin-membrane linkages at cell-cell junctions

Journal of Cellular Physiology
Rebecca Bakkevig SperryMarc D H Hansen

Abstract

Development is punctuated by morphogenetic rearrangements of epithelial tissues, including detachment of motile cells during epithelial-mesenchymal transition (EMT). Dramatic actin rearrangements occur as cell-cell junctions are dismantled and cells become independently motile during EMT. Characterizing dynamic actin rearrangements and identifying actin machinery driving these rearrangements is essential for understanding basic mechanisms of cell-cell junction remodeling. Using immunofluorescence and live cell imaging of scattering MDCK cells we examine dynamic actin rearrangement events during EMT and demonstrate that zyxin-VASP complexes mediate linkage of dynamic medial actin networks to adherens junction (AJ) membranes. A functional analysis of zyxin in EMT reveals its role in regulating disruption of actin membrane linkages at cell-cell junctions, altering cells' ability to fully detach and migrate independently during EMT. Expression of a constitutively active zyxin mutant results in persistent actin-membrane linkages and cell migration without loss of cell-cell adhesion. We propose zyxin functions in morphogenetic rearrangements, maintaining collective migration by transducing individual cells' movements through AJs, thu...Continue Reading

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Citations

Mar 29, 2016·Journal of Proteomics·Lorenzo E Hernández-CastellanoAndre M de Almeida
Sep 7, 2016·Biochemical and Biophysical Research Communications·Eliot LeeSoichiro Yamada
Jan 24, 2020·Scientific Reports·Ayşe KılıçScott T Weiss
Jul 15, 2018·The Journal of Cell Biology·William RazzellJennifer A Zallen
Oct 26, 2021·Frontiers in Physiology·Xuya KangJincai Luo

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